| Stress-induced activation of Nox contributes to cell survival signalling via production of hydrogen peroxide. | |
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MedLine Citation:
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PMID: 19344371 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reactive oxygen species (ROS) have traditionally been viewed as a toxic group of molecules; however, recent publications have shown that these molecules, including H(2)O(2), can also strongly promote cell survival. Even though the retina has a large capacity to produce ROS, little is known about its non-mitochondrial sources of these molecules, in particular the expression and function of NADPH oxidase (Nox) proteins which are involved in the direct generation of superoxide and indirectly H(2)O(2). This study demonstrated that 661W cells, a retina-derived cell line, and mouse retinal explants express Nox2, Nox4 and certain of their well-established regulators. The roles of Nox2 and Nox4 in producing pro-survival H(2)O(2) were determined using 661W cells and some of the controlling factors were identified. To ascertain if this phenomenon could have physiological relevance, the novel technique of time-lapse imaging of dichlorofluorescein fluorescence (generated upon H(2)O(2) production) in retinal explants was established and it showed that explants also produce a burst of H(2)O(2). The increase in H(2)O(2) production was partly blocked by an inhibitor of Nox proteins. Overall, this study demonstrates a pro-survival role of Nox2 and Nox4 in retina-derived cells, elucidates some of the regulatory mechanisms and reveals that a similar phenomenon exists in retinal tissue as a whole. |
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Authors:
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Gillian Groeger; Ashley M Mackey; Christopher A Pettigrew; Lavinia Bhatt; Thomas G Cotter |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-01 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 109 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-29 Completed Date: 2009-06-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1544-54 Citation Subset: IM |
Affiliation:
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Cell Development and Disease Laboratory, Biochemistry Department, Biosciences Institute, University College Cork, Cork, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
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pharmacology Animals Animals, Newborn Antioxidants / pharmacology Aristolochic Acids / pharmacology Cell Line Cell Survival / drug effects, physiology Culture Media, Serum-Free / pharmacology Dose-Response Relationship, Drug Eicosanoic Acids / pharmacology Flow Cytometry Gene Expression Regulation / drug effects, physiology Hydrogen Peroxide / metabolism*, pharmacology Mice Mice, Inbred C57BL NADH, NADPH Oxidoreductases / genetics, metabolism* Oxidative Stress / drug effects, physiology* Paraquat / pharmacology Photoreceptor Cells / drug effects, metabolism RNA, Small Interfering / pharmacology Reactive Oxygen Species / metabolism* Retina / cytology, metabolism* Signal Transduction / drug effects, physiology* Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Aristolochic Acids; 0/Culture Media, Serum-Free; 0/Eicosanoic Acids; 0/RNA, Small Interfering; 0/Reactive Oxygen Species; 149-45-1/1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; 313-67-7/aristolochic acid I; 4685-14-7/Paraquat; 506-30-9/arachidic acid; 7722-84-1/Hydrogen Peroxide; EC 1.6.-/NADH, NADPH Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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