Document Detail

Stress granules contribute to α-globin homeostasis in differentiating erythroid cells.
MedLine Citation:
PMID:  22452989     Owner:  NLM     Status:  MEDLINE    
Hemoglobin is the major biosynthetic product of developing erythroid cells. Assembly of hemoglobin requires the balanced production of globin proteins and the oxygen-carrying heme moiety. The heme-regulated inhibitor kinase (HRI) participates in this process by phosphorylating eIF2α and inhibiting the translation of globin proteins when levels of free heme are limiting. HRI is also activated in erythroid cells subjected to oxidative stress. Phospho-eIF2α-mediated translational repression induces the assembly of stress granules (SG), cytoplasmic foci that harbor untranslated mRNAs and promote the survival of cells subjected to adverse environmental conditions. We have found that differentiating erythroid, but not myelomonocytic or megakaryocytic, murine and human progenitor cells assemble SGs, in vitro and in vivo. Targeted knockdown of HRI or G3BP, a protein required for SG assembly, inhibits spontaneous and arsenite-induced assembly of SGs in erythroid progenitor cells. This is accompanied by reduced α-globin production and increased apoptosis suggesting that G3BP+ SGs facilitate the survival of developing erythroid cells.
Laura Ghisolfi; Shilpee Dutt; Marie E McConkey; Benjamin L Ebert; Paul Anderson
Related Documents :
3350149 - Biosynthesis of sialosyllactotetraosylceramide in human colorectal carcinoma cells.
6514189 - Reinnervation of the rat touch dome restores the merkel cell population reduced after d...
14648839 - Neurotrophin-3 signaling in mammalian merkel cell development.
7526039 - Oval cell lines oc/cde 6 and oc/cde 22 give rise to cholangio-cellular and undifferenti...
22886059 - Utilizing insulating nanoparticles as the spacer in laminated flexible polymer solar ce...
22303239 - The clickable guard cell, version ii: interactive model of guard cell signal transducti...
964979 - Malignant fibrillo-caveolated cell carcinoma of the human intestinal tract.
24893199 - Role of akt/mtorc1 pathway in pancreatic β-cell proliferation.
20569199 - Genomic tagging reveals a random association of endogenous ptdins5p 4-kinases iialpha a...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  420     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-23     Completed Date:  2012-06-22     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  768-74     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, One Jimmy Fund Way, Smith 652, Boston, MA 02115, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carrier Proteins / genetics,  metabolism
Cells, Cultured
Cytoplasmic Granules / metabolism,  physiology*
Erythroid Cells / cytology*,  metabolism
Mice, Inbred BALB C
Transcription, Genetic
alpha-Globins / biosynthesis*,  genetics
eIF-2 Kinase / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Carrier Proteins; 0/G3BP protein, human; 0/alpha-Globins; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Insulin transcriptionally regulates argininosuccinate synthase to maintain vascular endothelial func...
Next Document:  Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. ...