| Stress co-tolerance and trehalose content in baking strains of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 9079286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fourteen wild-type baking strains of Saccharomyces cerevisiae were grown in batch culture to true stationary phase (exogenous carbon source exhausted) and tested for their trehalose content and their tolerance to heat (52 degrees C for 4.5 min), ethanol (20% v/v for 30 min), H2O2 (0.3 M for 60 min), rapid freezing (-196 degrees C for 20 min, cooling rate 200 degrees C min-1), slow freezing (-20 degrees C for 24 h, cooling rate 3 degrees C min(-1)), salt (growth in 1.5 M NaCl agar) or acetic acid (growth in 0.4% w/v acetic acid agar) stresses. Stress tolerance among the strains was highly variable and up to 1000-fold differences existed between strains for some types of stress. Compared with previously published reports, all strains were tolerant to H2O2 stress. Correlation analysis of stress tolerance results demonstrated relationships between tolerance to H2O2 and tolerance to all stresses except ethanol. This may imply that oxidative processes are associated with a wide variety of cellular stresses and also indicate that the general robustness associated with industrial yeast may be a result of their oxidative stress tolerance. In addition, H2O2 tolerance might be a suitable marker for the general assessment of stress tolerance in yeast strains. Trehalose content failed to correlate with tolerance to any stress except acetic acid. This may indicate that the contribution of trehalose to tolerance to other stresses is either small or inconsistent and that trehalose may not be used as a general predictor of stress tolerance in true stationary phase yeast. |
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Authors:
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J G Lewis; R P Learmonth; P V Attfield; K Watson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: 18 ISSN: 1367-5435 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 1997 Jan |
Date Detail:
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Created Date: 1997-04-30 Completed Date: 1997-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 30-6 Citation Subset: B |
Affiliation:
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Department of Molecular and Cellular Biology, University of New England, Armidale, NSW, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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pharmacology Ethanol / pharmacology Freezing Heat-Shock Response Hydrogen Peroxide / pharmacology Oxidative Stress S Phase Saccharomyces cerevisiae / growth & development, metabolism* Salts / pharmacology Trehalose / analysis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Salts; 64-17-5/Ethanol; 64-19-7/Acetic Acid; 7722-84-1/Hydrogen Peroxide; 99-20-7/Trehalose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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