| Streptozotocin-resistant BRIN-BD11 cells possess wide spectrum of toxin tolerance and enhanced insulin-secretory capacity. | |
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MedLine Citation:
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PMID: 17992598 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since streptozotocin (STZ) exhibits beta-cell toxicity, mediated through diverse mechanisms, multiple toxin resistance can be expected in insulin-secretory cells rendered STZ-resistant. RINm5F, but not all cell lines surviving STZ treatment, possess higher insulin content than native parental cells and additional tolerance against alloxan. To understand the impact of STZ tolerant cell selection on toxin resistance and insulin-secretory function, STZ-resistant BRIN-BD11 cells were generated by iterative acute exposure to 20 mM STZ. These cells, denoted BRINst cells, exhibited resistance to toxic challenges from STZ, H(2)O(2), and ninhydrin. Insulin content and both glucose and arginine-stimulated insulin secretion were significantly enhanced in BRINst cells. The toxin-resistance of BRINst cells was gradually lost during continuous cultivation without STZ challenge. However, enhanced insulin secretory capacity at high passage in BRINst cells persisted. Although total SOD activity was decreased, catalase activity was increased and appeared to be important for the ninhydrin and STZ resistance of BRINst cells. This was associated with reductions of both STZ- and ninhydrin-induced DNA damage, although DNA repair was abolished. Further characterization of cells exhibiting multiple toxin tolerance and an enhanced insulin secretory function could provide useful lessons for understanding of beta-cell survival. |
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Authors:
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Hui-Kang Liu; Jane T McCluskey; Neville H McClenghan; Peter R Flatt |
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Publication Detail:
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Type: Journal Article Date: 2007-09-29 |
Journal Detail:
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Title: Endocrine Volume: 32 ISSN: 1355-008X ISO Abbreviation: Endocrine Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-11-09 Completed Date: 2008-03-27 Revised Date: 2010-06-24 |
Medline Journal Info:
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Nlm Unique ID: 9434444 Medline TA: Endocrine Country: United States |
Other Details:
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Languages: eng Pagination: 20-9 Citation Subset: IM |
Affiliation:
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School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, Northern Ireland, UK. hk.liu@nricm.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / pharmacology Antioxidants / metabolism* Cell Culture Techniques DNA Damage DNA Repair / physiology* Drug Resistance, Multiple / physiology* Glucose / metabolism Hydrogen Peroxide / pharmacology Insulin / biosynthesis, secretion Insulin-Secreting Cells / metabolism*, secretion Ninhydrin / pharmacology Oxidants / pharmacology Radiation-Sensitizing Agents / pharmacology Rats Signal Transduction / physiology* Streptozocin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Antioxidants; 0/Oxidants; 0/Radiation-Sensitizing Agents; 11061-68-0/Insulin; 18883-66-4/Streptozocin; 485-47-2/Ninhydrin; 50-99-7/Glucose; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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