Document Detail


Streptococcus suis capsular polysaccharide inhibits phagocytosis through destabilization of lipid microdomains and prevents lactosylceramide-dependent recognition.
MedLine Citation:
PMID:  22124659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus suis type 2 is a major swine pathogen and a zoonotic agent, causing meningitis in both swine and humans. S. suis infects the host through the respiratory route, reaches the bloodstream, and persists until breaching into the central nervous system. The capsular polysaccharide (CPS) of S. suis type 2 is considered a key virulence factor of the bacteria. Though CPS allows S. suis to adhere to the membrane of cells of the immune system, it provides protection against phagocytosis. In fact, nonencapsulated mutants are easily internalized and killed by macrophages and dendritic cells. The objective of this work was to study the molecular mechanisms by which the CPS of S. suis prevents phagocytosis. By using latex beads covalently linked with purified CPS, it was shown that CPS itself was sufficient to inhibit entry of both latex beads and bystander fluorescent beads into macrophages. Upon contact with macrophages, encapsulated S. suis was shown to destabilize lipid microdomains at the cell surface, to block nitric oxide (NO) production during infection, and to prevent lactosylceramide accumulation at the phagocytic cup during infection. In contrast, the nonencapsulated mutant was easily internalized via lipid rafts, in a filipin-sensitive manner, leading to lactosylceramide recruitment and strong NO production. This is the first report to identify a role for CPS in lipid microdomain stability and to recognize an interaction between S. suis and lactosylceramide in phagocytes.
Authors:
Mathieu Houde; Marcelo Gottschalk; Fleur Gagnon; Marie-Rose Van Calsteren; Mariela Segura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-28
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-03-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  506-17     Citation Subset:  IM    
Affiliation:
Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology
Antigens, CD / metabolism*
Bacterial Adhesion
Carbohydrate Conformation
Cells, Cultured
Female
Filipin / pharmacology
Gene Expression Regulation, Bacterial / physiology
Lactosylceramides / metabolism*
Macrophages / cytology,  physiology
Membrane Microdomains / drug effects*
Mice
Microspheres
Phagocytosis / drug effects*
Polysaccharides, Bacterial / chemistry,  metabolism*,  pharmacology*
Streptococcus suis / drug effects,  metabolism*,  pathogenicity
Virulence
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antigens, CD; 0/Lactosylceramides; 0/Polysaccharides, Bacterial; 4682-48-8/CDw17 antigen; 480-49-9/Filipin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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