Document Detail


Streptococcus pyogenes cytolysin-mediated translocation does not require pore formation by streptolysin O.
MedLine Citation:
PMID:  20339385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial toxin injection into the host cell is required for the virulence of numerous pathogenic bacteria. Cytolysin-mediated translocation (CMT) of Streptococcus pyogenes uses streptolysin O (SLO) to translocate the S. pyogenes nicotinamide adenine dinucleotide-glycohydrolase (SPN) into the host cell cytosol, resulting in the death of the host cell. Although SLO is a pore-forming protein, previous studies have shown that pore formation alone is not sufficient for CMT to occur. Thus, the role and requirement of the SLO pore remains unclear. In this study, we constructed various S. pyogenes strains expressing altered forms of SLO to assess the importance of pore formation. We observed that SLO mutants that are unable to form pores retain the ability to translocate SPN. In addition, SPN translocation occurs after inhibition of actin polymerization, suggesting that CMT occurs independently of clathrin-mediated endocytosis. Moreover, despite the ability of mutants to translocate SPN, their cytotoxic effect requires SLO pore formation.
Authors:
N'Goundo Magassa; Sukantha Chandrasekaran; Michael G Caparon
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-03-26
Journal Detail:
Title:  EMBO reports     Volume:  11     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-29     Completed Date:  2010-07-27     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  400-5     Citation Subset:  IM    
Affiliation:
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110-1093, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry,  metabolism,  ultrastructure
Cell Death
Cell Line, Tumor
Cell Membrane / metabolism
Humans
Mutation / genetics
Porins / metabolism*,  ultrastructure
Protein Structure, Quaternary
Protein Transport
Streptococcus pyogenes / metabolism*
Streptolysins / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
5T32AI007172/AI/NIAID NIH HHS; AI064721/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Porins; 0/Streptolysins; 0/streptolysin O
Comments/Corrections

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