Document Detail


Streptococcus pneumoniae induces exocytosis of Weibel-Palade bodies in pulmonary endothelial cells.
MedLine Citation:
PMID:  21999205     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Invasive pneumococcal infections due to Streptococcus pneumoniae lead to inflammatory infiltration of leukocytes into lung alveolus, meninges, and to septic dissemination within the vascular system. The lung microvasculature is covered by pulmonary endothelial cells containing Weibel-Palade bodies (WPB) releasing procoagulant von Willebrand factor (vWF) and other proteins in response to inflammatory stimuli. The influence of pathogenic pneumococci on secretion of WPB-proteins is unknown. Here, we report that adherence of S. pneumoniae to primary human pulmonary microvascular endothelial cells (HPMEC) stimulates the WPB exocytosis and the secretion of vWF and interleukin 8 (IL-8). Moreover, infection analyses performed with pneumococcal mutants deficient in the expression of cytotoxic pneumolysin demonstrated that, in addition to direct bacterial adherence, sublytic concentrations of pneumolysin stimulated vWF secretion. The release of vWF was induced after infection with pneumococci from both the apical and the basal cell surfaces, which implies a stimulation of WPB exocytosis in both directions: From inside the vasculature and also following invasive pneumococcal transmigration from pulmonary tissue into the bloodstream. In conclusion, this study demonstrates that the most relevant pulmonary pathogen S. pneumoniae induces release of pro-inflammatory and pro-coagulative components directly contributing to pathophysiological processes leading to fatal tissue injury during course of infection.
Authors:
Melanie Lüttge; Marcus Fulde; Susanne R Talay; Andreas Nerlich; Manfred Rohde; Klaus T Preissner; Sven Hammerschmidt; Michael Steinert; Tim J Mitchell; Gursharan S Chhatwal; Simone Bergmann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-14
Journal Detail:
Title:  Cellular microbiology     Volume:  -     ISSN:  1462-5822     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, Germany, Centre for Infection Medicine, Institute for Microbiology, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany, Department of Biochemistry, Medical School, Justus-Liebig-University, Friedrichstrasse 24, 35392 Giessen, Germany, Department of Genetics of Microorganisms, Institute for Genetics and Functional Genomics, Ernst Moritz Arndt University of Greifswald, Friedrich-Ludwig-Jahn-Strasse 15a, 17487 Greifswald, Germany, Department of Infection Biology, Institute of Microbiology, Technische Universität Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany, Institute of Infection, Immunity and Inflammation, University of Glasgow, G12 8TA, Scotland, UK.
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