| Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth. | |
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MedLine Citation:
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PMID: 22311922 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanisms by which Streptococcus pneumoniae obtains carbohydrates for growth during airway colonization remain to be elucidated. The low concentration of free carbohydrates in the normal human airway suggests that pneumococci must utilize complex glycan structures for growth. The glycosaminoglycan hyaluronic acid is present on the apical surface of airway epithelial cells. As pneumococci express a hyaluronate lyase (Hyl) that cleaves hyaluronic acid into disaccharides, we hypothesized that during colonization pneumococci utilize the released carbohydrates for growth. Hyaluronic acid supported significant pneumococcal growth in an hyl-dependent manner. A phosphoenolpyruvate-dependent phosphotransferase system (PTS) and an unsaturated glucuronyl hydrolase (Ugl) encoded downstream of hyl are also essential for growth on hyaluronic acid. This genomic arrangement is present in several other organisms, suggesting conservation of the utilization mechanism between species. In vivo experiments support the hypothesis that S. pneumoniae utilizes hyaluronic acid as a carbon source during colonization. We also demonstrate that pneumococci can utilize the hyaluronic acid capsule of other bacterial species for growth, suggesting an alternative carbohydrate source for pneumococcal growth. Together, these data support a novel function for pneumococcal degradation of hyaluronic acid in vivo and provide mechanistic details of growth on this glycosaminoglycan. |
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Authors:
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Carolyn Marion; Jason M Stewart; Mia F Tazi; Amanda M Burnaugh; Caroline M Linke; Shireen A Woodiga; Samantha J King |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-02-06 |
Journal Detail:
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Title: Infection and immunity Volume: 80 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-19 Completed Date: 2012-05-10 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 1390-8 Citation Subset: IM |
Affiliation:
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Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / genetics, metabolism Glycoside Hydrolases / metabolism Humans Hyaluronic Acid / metabolism* Mice Phosphotransferases / metabolism Polysaccharide-Lyases / metabolism Streptococcus pneumoniae / enzymology, growth & development, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1R01AI076341/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 9004-61-9/Hyaluronic Acid; EC 2.7.-/Phosphotransferases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/unsaturated glucuronyl hydrolase; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.1/hyaluronate lyase |
| Comments/Corrections | |
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