Document Detail


Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth.
MedLine Citation:
PMID:  22311922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanisms by which Streptococcus pneumoniae obtains carbohydrates for growth during airway colonization remain to be elucidated. The low concentration of free carbohydrates in the normal human airway suggests that pneumococci must utilize complex glycan structures for growth. The glycosaminoglycan hyaluronic acid is present on the apical surface of airway epithelial cells. As pneumococci express a hyaluronate lyase (Hyl) that cleaves hyaluronic acid into disaccharides, we hypothesized that during colonization pneumococci utilize the released carbohydrates for growth. Hyaluronic acid supported significant pneumococcal growth in an hyl-dependent manner. A phosphoenolpyruvate-dependent phosphotransferase system (PTS) and an unsaturated glucuronyl hydrolase (Ugl) encoded downstream of hyl are also essential for growth on hyaluronic acid. This genomic arrangement is present in several other organisms, suggesting conservation of the utilization mechanism between species. In vivo experiments support the hypothesis that S. pneumoniae utilizes hyaluronic acid as a carbon source during colonization. We also demonstrate that pneumococci can utilize the hyaluronic acid capsule of other bacterial species for growth, suggesting an alternative carbohydrate source for pneumococcal growth. Together, these data support a novel function for pneumococcal degradation of hyaluronic acid in vivo and provide mechanistic details of growth on this glycosaminoglycan.
Authors:
Carolyn Marion; Jason M Stewart; Mia F Tazi; Amanda M Burnaugh; Caroline M Linke; Shireen A Woodiga; Samantha J King
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-02-06
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-19     Completed Date:  2012-05-10     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1390-8     Citation Subset:  IM    
Affiliation:
Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics,  metabolism
Glycoside Hydrolases / metabolism
Humans
Hyaluronic Acid / metabolism*
Mice
Phosphotransferases / metabolism
Polysaccharide-Lyases / metabolism
Streptococcus pneumoniae / enzymology,  growth & development,  metabolism*
Grant Support
ID/Acronym/Agency:
1R01AI076341/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 9004-61-9/Hyaluronic Acid; EC 2.7.-/Phosphotransferases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/unsaturated glucuronyl hydrolase; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.1/hyaluronate lyase
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