Document Detail


Streptococcus mutans inhibits Candida albicans hyphal formation by the fatty acid signaling molecule trans-2-decenoic acid (SDSF).
MedLine Citation:
PMID:  20572249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the human mouth, fungi and several hundred species of bacteria coexist. Here we report a case of interkingdom signaling in the oral cavity: A compound excreted by the caries bacterium Streptococcus mutans inhibits the morphological transition from yeast to hyphae, an important virulence trait, in the opportunistic fungus Candida albicans. The compound excreted by S. mutans was originally studied because it inhibited signaling by the universal bacterial signal autoinducer-2 (AI-2), determined by the luminescence of a Vibrio harveyi sensor strain. The inhibitor was purified from cell-free culture supernatants of S. mutans guided by its activity. Its chemical structure was elucidated by using NMR spectroscopy and GC-MS and proved to be trans-2-decenoic acid. We show that trans-2-decenoic acid does not inhibit AI-2-specific signaling, but rather the luciferase reaction used for its detection. A potential biological role of trans-2-decenoic acid was then discovered. It is able to suppress the transition from yeast to hyphal morphology in the opportunistic human pathogen Candida albicans at concentrations that do not affect growth. The expression of HWP1, a hyphal-specific signature gene of C. albicans, is abolished by trans-2-decenoic acid. trans-2-Decenoic acid is structurally similar to the diffusible signal factor (DSF) family of interkingdom-signaling molecules and is the first member of this family from a Gram-positive organism (Streptococcus DSF, SDSF). SDSF activity was also found in S. mitis, S. oralis, and S. sanguinis, but not in other oral bacteria. SDSF could be relevant in shaping multispecies Candida bacteria biofilms in the human body.
Authors:
Ramiro Vílchez; André Lemme; Britta Ballhausen; Verena Thiel; Stefan Schulz; Rolf Jansen; Helena Sztajer; Irene Wagner-Döbler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  11     ISSN:  1439-7633     ISO Abbreviation:  Chembiochem     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1552-62     Citation Subset:  IM    
Affiliation:
Helmholtz-Center for Infection Research (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany.
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MeSH Terms
Descriptor/Qualifier:
Biofilms
Candida albicans / ultrastructure*
Fatty Acids / pharmacology
Fatty Acids, Monounsaturated / pharmacology*
Humans
Hyphae / drug effects,  growth & development*
Mouth / microbiology
Signal Transduction*
Streptococcus mutans / physiology*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 3913-85-7/2-decenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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