Document Detail

Streptococcal pyrogenic exotoxin B induces apoptosis and reduces phagocytic activity in U937 cells.
MedLine Citation:
PMID:  9864206     Owner:  NLM     Status:  MEDLINE    
Treatment of U937 human monocyte-like cells with Streptococcus pyogenes led to an induction of apoptosis in these cells. A comparison between the wild-type strain and its isogenic protease-negative mutant indicated that the production of streptococcal pyrogenic exotoxin B (SPE B), a cysteine protease, caused a greater extent of apoptosis in U937 cells. Further study using purified SPE B showed that this protease alone could induce U937 cells to undergo apoptosis, which was characterized by morphologic changes, DNA fragmentation laddering on the gel, and an increase in the percentages of hypodiploid cells. The protease activity of SPE B was required for apoptosis to proceed, since treatment with cysteine protease inhibitor E64 or heat inactivation abrogated this death-inducing effect. The SPE B-induced apoptosis pathway was interleukin-1beta converting enzyme (ICE) family protease dependent. Further experiments showed that the phagocytic activity of U937 cells was reduced by SPE B. Treatment with E64 and heat inactivation both abrogated this phagocytosis-inhibitory effect. Taken together, the present data show that SPE B not only possesses the ability to induce apoptosis in monocytic cells but also helps bacteria to resist phagocytosis by host cells.
C F Kuo; J J Wu; P J Tsai; F J Kao; H Y Lei; M T Lin; Y S Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  67     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  126-30     Citation Subset:  IM    
Department of Microbiology, National Cheng Kung University Medical College, Taiwan, Republic of China.
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MeSH Terms
Apoptosis / immunology*
Bacterial Proteins*
Cysteine Proteinase Inhibitors / pharmacology
Exotoxins / genetics,  physiology*
Hot Temperature
Immunosuppressive Agents / pharmacology*
Membrane Proteins*
Mutagenesis, Site-Directed
Phagocytosis / immunology*
Pyrogens / physiology*
Streptococcus pyogenes / enzymology,  immunology*
U937 Cells
Reg. No./Substance:
0/Bacterial Proteins; 0/Cysteine Proteinase Inhibitors; 0/Exotoxins; 0/Immunosuppressive Agents; 0/Membrane Proteins; 0/Pyrogens; 0/SpeA protein, Streptococcus pyogenes; 0/erythrogenic toxin

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