Document Detail


Streptococcal erythrogenic toxin type C is not a phosphorylated protein. Description of two different purification procedures and investigation of its phosphorylation state.
MedLine Citation:
PMID:  7920465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythrogenic toxin type C (ETC) from different streptococcal group A strains was successively purified by absorption on phenylsepharose, acidic dialysis of the eluate at 40% saturated ammonium sulphate solution, CM-Sepharose chromatography, finally by immunoaffinity chromatography on monoclonal antibodies. Second, after growing of bacteria in the presence of [32P]orthophosphate to phosphorylate ETC, the ETC was purified with phenylsepharose following immunoaffinity chromatography. The occurrence of phosphoamino acids in the purified ETC was investigated by an immunoassay. No phosphoamino acids could be detected in the ETC molecule. Also after radiolabelling with 32P it was not possible to demonstrate a radioactive signal. The treatment with alkaline phosphatase has no influence on the mitogenicity or position of ETC in isoelectric focusing. The results obtained led to the conclusion that in contrast to the literature, ETC is not a phosphorylated protein.
Authors:
J H Ozegowski; L Wollweber; K H Schmidt; S Vettermann; W Reichardt; W Köhler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  9     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-11-08     Completed Date:  1994-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-76     Citation Subset:  IM    
Affiliation:
Institut für Experimentelle Mikrobiologie der Friedrich-Schiller-Universität, Jena, FRG.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Bacterial Proteins / immunology,  isolation & purification*
Chromatography, Affinity
Chromatography, Agarose
Exotoxins / immunology,  isolation & purification*
Humans
Lymphocyte Activation
Membrane Proteins*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Phosphorylation
Streptococcus pyogenes / metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bacterial Proteins; 0/Exotoxins; 0/Membrane Proteins; 0/SpeA protein, Streptococcus pyogenes; 0/erythrogenic toxin

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