Document Detail


Strenuous exercise-induced change in redox state of human serum albumin during intensive kendo training.
MedLine Citation:
PMID:  12139771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) using an ion-exchange (DEAE-form) column shows three components: The principal component corresponds to human mercaptalbumin (HMA); the secondary to nonmercaptalbumin (HNA), having mixed disulfide with cystine (HNA[Cys]), or oxidized glutathione (HNA[Glut]); and the tertiary to HNA, oxidized more highly than mixed disulfide. The purpose of the present study is to clarify the effects of strenuous exercise load on HMA--><--HNA conversion (i.e., dynamic change in redox state) of HSA from elite kendo athletes (n=30; 20.0+/-1.1 years old). They participated in an intensive kendo training camp for 5 d. The mean value for the HMA fraction (f[HMA]) of kendo athletes after camp (62.8+/-2.4%) was significantly lower than before camp (71.9+/-3.7%) (p<0.0005). In contrast, the mean value for f(HNA-1) (i.e., f[HNA(Cys) and HNA(Glut)]) after camp (34.2+/-2.1%) was significantly higher than before camp (25.7+/-3.7%) (p<0.0005). These results suggested that strenuous physical exercise markedly increased the oxidized albumin level in extracellular fluids during the intensive training camp.
Authors:
Hajime Imai; Tomoya Hayashi; Tsuneo Negawa; Koji Nakamura; Mihoko Tomida; Kunihide Koda; Tomio Tajima; Yasuko Koda; Kazuhiro Suda; Seiichi Era
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of physiology     Volume:  52     ISSN:  0021-521X     ISO Abbreviation:  Jpn. J. Physiol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-07-25     Completed Date:  2003-02-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985184R     Medline TA:  Jpn J Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  135-40     Citation Subset:  IM    
Affiliation:
Department of Health and Physical Education, Faculty of Education, Gifu University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Chromatography, High Pressure Liquid
Extracellular Space / metabolism
Humans
Male
Martial Arts / physiology*
Oxidation-Reduction
Physical Exertion / physiology*
Serum Albumin / metabolism*
Sulfhydryl Compounds / metabolism
Chemical
Reg. No./Substance:
0/Serum Albumin; 0/Sulfhydryl Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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