Document Detail

Strategy for photostable proximity bioassays using lanthanides.
MedLine Citation:
PMID:  17356638     Owner:  NLM     Status:  MEDLINE    
We report initial findings for research aimed at creating photostable lanthanide chelate reporters for proximity assays. These reporters take advantage of the nanometer-scale distance dependence of fluorescence enhancement for molecules in the vicinity of noble metal nanoparticles and also capitalize on some unique properties of lanthanide chelates. This approach promises to lead to proximity assays that do not suffer from photobleaching and offer very high on/off enhancement ratios. Results for lanthanide chelates on silver island films and in colloidal suspensions are reported. Enhancement factors range from 1 to 2 orders of magnitude, with larger enhancements for strongly quenched lanthanides.
Jeanne P Haushalter; Gregory W Faris
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Applied optics     Volume:  46     ISSN:  0003-6935     ISO Abbreviation:  Appl Opt     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-14     Completed Date:  2007-05-09     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0247660     Medline TA:  Appl Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1918-23     Citation Subset:  IM    
Molecular Physics Laboratory, SRI International, CA 94025, USA.
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MeSH Terms
Biological Assay / methods*
Drug Stability
Lanthanoid Series Elements / chemistry*,  radiation effects
Photochemistry / methods
Spectrometry, Fluorescence / methods*
Surface Plasmon Resonance / methods*
Grant Support
R21 AI055728-01/AI/NIAID NIH HHS; R21 AI055728-02/AI/NIAID NIH HHS; R21AI055728/AI/NIAID NIH HHS
Reg. No./Substance:
0/Lanthanoid Series Elements

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