| Strategy for photostable proximity bioassays using lanthanides. | |
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MedLine Citation:
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PMID: 17356638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report initial findings for research aimed at creating photostable lanthanide chelate reporters for proximity assays. These reporters take advantage of the nanometer-scale distance dependence of fluorescence enhancement for molecules in the vicinity of noble metal nanoparticles and also capitalize on some unique properties of lanthanide chelates. This approach promises to lead to proximity assays that do not suffer from photobleaching and offer very high on/off enhancement ratios. Results for lanthanide chelates on silver island films and in colloidal suspensions are reported. Enhancement factors range from 1 to 2 orders of magnitude, with larger enhancements for strongly quenched lanthanides. |
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Authors:
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Jeanne P Haushalter; Gregory W Faris |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Applied optics Volume: 46 ISSN: 0003-6935 ISO Abbreviation: Appl Opt Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-14 Completed Date: 2007-05-09 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0247660 Medline TA: Appl Opt Country: United States |
Other Details:
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Languages: eng Pagination: 1918-23 Citation Subset: IM |
Affiliation:
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Molecular Physics Laboratory, SRI International, CA 94025, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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methods* Drug Stability Lanthanoid Series Elements / chemistry*, radiation effects Light Photochemistry / methods Spectrometry, Fluorescence / methods* Surface Plasmon Resonance / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R21 AI055728-01/AI/NIAID NIH HHS; R21 AI055728-02/AI/NIAID NIH HHS; R21AI055728/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lanthanoid Series Elements |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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