Document Detail


A Strategy for Identifying Noncoding RNAs Using Whole-Genome Tiling Arrays.
MedLine Citation:
PMID:  22735996     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Whole-genome tiling arrays are powerful tools for detecting and characterizing novel RNA transcripts. Here, we describe a complete method combining elements of molecular and computational biology to identify small noncoding RNA (sRNA) transcripts. We focus on the key features of this approach, which include size-fractionation of input RNA, direct detection of array hybridization with antibodies that recognize RNA:DNA hybrids, and correlation-based computational methods for automated sRNA identification and boundary determination.
Authors:
Stephen G Landt; Eduardo Abeliuk
Related Documents :
22675536 - Characterization of a conserved interaction between dna glycosylase and para in mycobac...
22456976 - Analysis of the dna damage produced by a platinum-acridine antitumor agent and its effe...
22535986 - Accurate identification of candida parapsilosis (sensu lato) by use of mitochondrial dn...
22412956 - Dna barcoding and microsatellites help species delimitation and hybrid identification i...
19204896 - Dna recognition by the salmonella enterica serovar typhimurium transcription factor slya.
22195966 - Dynamics of histone h3 deposition in vivo reveal a nucleosome gap-filling mechanism for...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  905     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  29-39     Citation Subset:  IM    
Affiliation:
Department of Genetics, Stanford University, Stanford, CA, USA, sglandt@gmail.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  How to Identify CRISPRs in Sequencing Data.
Next Document:  Genetic Screens to Identify Bacterial sRNA Regulators.