| Strategies for measurements of pseudocontact shifts in protein NMR spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 17910025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Paramagnetic metal ions bound to proteins generate a dipolar field that can be accurately probed by pseudocontact shifts (PCS) displayed by the protein's nuclear spins. PCS are highly useful for determining the coordinates of individual spins in the molecule and for rapid structure determinations of entire protein-protein and protein-ligand complexes. However, PCS measurements require reliable resonance assignments for the molecule in its paramagnetic state and in a diamagnetic reference state. This article discusses different approaches for pairwise resonance assignments, with emphasis on a strategy which exploits chemical exchange between the two states. |
| | |
Authors:
|
Michael John; Gottfried Otting |
Related Documents
:
|
16719475 - Increasing the accuracy of solution nmr structures of membrane proteins by application ... 11785755 - Thermodynamic insights into proteins from nmr spin relaxation studies. 12783525 - Detection of short-lived transient protein-protein interactions by intermolecular nucle... 20838855 - High-resolution methyl edited gft nmr experiments for protein resonance assignments and... 16053445 - High-affinity interaction of the n-terminal myristoylation motif of the neuronal calciu... 15647305 - On the quality of tree-based protein classification. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Chemphyschem : a European journal of chemical physics and physical chemistry Volume: 8 ISSN: 1439-4235 ISO Abbreviation: Chemphyschem Publication Date: 2007 Nov |
Date Detail:
|
Created Date: 2007-11-07 Completed Date: 2007-12-11 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100954211 Medline TA: Chemphyschem Country: Germany |
Other Details:
|
Languages: eng Pagination: 2309-13 Citation Subset: IM |
Affiliation:
|
Institut für Anorganische Chemie, Georg August Universität, Tammannstrasse 4, 37073 Göttingen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Metalloproteins
/
chemistry* Models, Molecular Nuclear Magnetic Resonance, Biomolecular / methods* Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Metalloproteins; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IR and NMR properties of ionic liquids: do they tell us the same thing?
Next Document: Quantitative ATP synthesis in human liver measured by localized 31P spectroscopy using the magnetiza...