Document Detail


Strategies for the cryopreservation of microencapsulated cells.
MedLine Citation:
PMID:  14705003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The major challenge in developing cryopreservation protocols for microencapsulated cells is that the relatively large size (300-400 microm) and the fragile semipermeable membrane of microcapsules makes them particularly prone to cryodamage. Rapid-cooling cryopreservation protocols with high DMSO concentrations (3.5M, 25% v/v) resulted in low post-thaw cell viability (<10%), which did not improve with higher concentrations (4.5M, 32% v/v) and longer exposure to DMSO, even though the majority of microcapsules (60-80%) remained intact. Subsequent investigations of slow cooling with a range of DMSO and EG concentrations resulted in a much higher post-thaw cell viability (80-85%), with the majority of the microcapsules remaining intact ( approximately 60%) when DMSO was used at a concentration of 2.8M (20% v/v) and EG at a concentration of 2.7M (15% v/v). The presence of 0.25M sucrose significantly improved post-thaw cell viability upon slow cooling with 2.8M (20% v/v) DMSO, although it had no effect on microcapsule integrity. Multistep exposure and removal of sucrose did not significantly improve either post-thaw cell viability or microcapsule integrity, compared to a single-step protocol. Ficoll 20% (w/v) also did not significantly improve post-thaw cell viability and microcapsule integrity. Hence, the optimal condition for microcapsule cryopreservation developed in this study is slow cooling with 2.8M (20% v/v) DMSO and 0.25M sucrose.
Authors:
Boon Chin Heng; Hanry Yu; Soon Chye Ng
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  85     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-05     Completed Date:  2004-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  202-13     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc.
Affiliation:
Department of Obstetrics & Gynaecology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore. obgngsc@nus.edu.sg
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Survival / drug effects,  physiology
Cercopithecus aethiops
Coated Materials, Biocompatible / chemistry
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Dimethyl Sulfoxide / chemistry,  pharmacology*
Ethylene Glycol / chemistry,  pharmacology*
Kidney / chemistry,  cytology*,  drug effects,  physiology*
Membranes, Artificial
Microspheres
Particle Size
Specimen Handling / methods
Sucrose / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Cryoprotective Agents; 0/Membranes, Artificial; 107-21-1/Ethylene Glycol; 57-50-1/Sucrose; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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