Document Detail


Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes.
MedLine Citation:
PMID:  70463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Requirements for flow cytometry of metaphase chromosomes stained with three deoxyribonucleic acid (DNA)-specific fluorescent dyes--Hoechst 33258, Chromomycin A3, and ethidium bromide--are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented that exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A3 to ethidium bromide when simultaneously bound to hamster mitotic cells.
Authors:
R H Jensen; R G Langlois; B H Mayall
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  954-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Chromomycins
Chromosomes / analysis*
Cricetinae
DNA / analysis*
Ethidium
Fluorescent Dyes
Mitosis
Photometry*
Spectrometry, Fluorescence
Staining and Labeling*
Chemical
Reg. No./Substance:
0/Chromomycins; 0/Fluorescent Dyes; 3546-21-2/Ethidium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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