| Strain-level typing and identification of bacteria using mass spectrometry-based proteomics. | |
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MedLine Citation:
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PMID: 22452665 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Due to the alarming expansion in the diversity and occurrence of bacteria displaying virulence and resistance to anti-microbial agents, it is increasingly important to be able to detect these microorganisms and to differentiate and identify closely related species, as well as different strains of a given species. In this study, a mass spectrometry proteomics approach is applied, exploiting lipid-based protein immobilization (LPI), wherein intact bacterial cells are bound, via membrane-gold interactions, within a FlowCell. The bound cells are subjected to enzymatic digestion for the generation of peptides, which are subsequently identified, using LC-MS. Following database matching, strain-specific peptides are used for sub-species-level discrimination. The method is shown to enable a reliable typing and identification of closely related strains of the same bacterial species, herein illustrated for Helicobacter pylori. |
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Authors:
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Roger Karlsson; Max Davidson; Liselott Svensson-Stadler; Anders Karlsson; Kenneth Olesen; Elisabet Carlsohn; Edward R Moore |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-27 |
Journal Detail:
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Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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