| Strain Differences in Alveolar Neutrophil Infiltration and Macrophage Phenotypes in an Acute Lung Inflammation Model. | |
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MedLine Citation:
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PMID: 21541443 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Pulmonary infection is a major cause of mortality and morbidity, and the magnitude of the lung inflammatory response correlates with patient survival. Previously, we have shown that neutrophil migration into joints is regulated by arthritis severity quantitative trait loci (QTLs). However, it is unclear whether these QTLs contribute to the regulation of lung inflammation in pneumonias. Therefore, in this study, we examined two inbred rat strains that differ in these QTLs and their susceptibility to joint inflammation, DA and F344, to more clearly define the factors regulating acute inflammatory responses in the lung. Staphylococcal cell wall components lipoteichoic acid (LTA) and peptidoglycan (PGN), administered intratracheally, significantly increased the numbers of neutrophils retrieved in the bronchoalveolar lavage fluid (BALF). F344 had approximately ten fold more neutrophils in the BALF compared to DA (p<0.001) and higher BALF concentrations of total protein, TNF-α, and MIP-2. LTA/PGN administration in DA×F344 congenic strains (Cia3d, Cia4, Cia5a, and Cia6) resulted in inflammation similar to that in DA, demonstrating that the genes responsible for the differences in pulmonary inflammation are not contained within the chromosomal intervals carried by these congenic strains. Alveolar macrophages (AM) isolated from naïve F344 stimulated in vitro with LTA/PGN produced significantly higher levels of KC and MIP-2 than AM from DA rats. The differences were related to differential MAPK phosphorylation. We conclude that the factors contributing to inflammation can be site and challenge dependent. A better understanding of site specific inflammation may lead to more effective treatment of acute lung inflammation and injury. |
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Authors:
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Yinzhong Zhang; Xinchun Lin; Kiyokazu Koga; Koichiro Takahashi; Helena M Linge; Adriana Mello; Teresina Laragione; Percio S Gulko; Edmund J Miller |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-28 |
Journal Detail:
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Title: Molecular medicine (Cambridge, Mass.) Volume: - ISSN: 1528-3658 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-5-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9501023 Medline TA: Mol Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Center for Heart and Lung Research, The Feinstein Institute for Medical Research, Manhasset, New York 11030, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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