Document Detail

Storage of red blood cells affects membrane composition, microvesiculation, and in vitro quality.
MedLine Citation:
PMID:  23320518     Owner:  NLM     Status:  Publisher    
BACKGROUND: During storage detrimental biochemical and biomechanical changes occur within a red blood cell (RBC). RBC microparticles (RMPs) produced during storage have been identified as biomarkers of RBC quality, being potentially immunogenic and inhibitory to nitric oxide regulation. STUDY DESIGN AND METHODS: In this study, microvesiculation and changes in the composition of the RBC membrane were investigated throughout 49 days of storage and were correlated with in vitro assays examining membrane quality. Leukoreduced RBC units produced using the buffy coat method were collected and stored at 1 to 6°C and were tested weekly for hemolysis, osmotic fragility, deformability, ATP, hematologic indices, and morphology. Microvesiculation was assessed using multicolor flow cytometry. High-performance liquid chromatography and mass spectrometry were used to determine the composition and quantity of phospholipids (PLs) and cholesterol (C) on Days 2 and 43. RESULTS: The assessment of RBCs throughout storage revealed significant increases in percent hemolysis, while significant decreases in ATP concentrations, and the mean corpuscular hemoglobin concentration were observed. Flow cytometry analysis revealed a significant increase in the mean number of microparticles per microliter during storage. Throughout storage, significant decreases were identified in the amount of PLs and total lipids within the RBC membrane. No significant change in the amount of C in the RBC membrane was identified. CONCLUSION: Significant changes to the RBC membrane occur during storage. The length of storage will influence RMP generation, osmotic fragility, hemolysis, and changes in deformability. These changes in RBC in vitro quality may contribute to transfusion reactions and negative posttransfusion outcomes.
Ruqayyah Almizraq; Jayme D R Tchir; Jelena L Holovati; Jason P Acker
Related Documents :
12689238 - Dislocation lines as the precursor of the melting of crystalline solids observed in mon...
24068328 - Lamp2 as a marker of ebv-mediated b lymphocyte transformation in the study of lysosomal...
11414908 - Condensation of hard rods under gravity: exact results in one dimension.
1240758 - Physical studies on phosphonium phosphatidylcholine. a unique [31p]phosphorus nuclear-m...
23227828 - A renewable and superior thermal resistant cellulose-based composite nonwoven as lithiu...
24467298 - Enhanced charge transport kinetics in anisotropic, stratified photoanodes.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Transfusion     Volume:  -     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 American Association of Blood Banks.
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; Canadian Blood Services, Research and Development, Edmonton, Alberta, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Air ions and mood outcomes: a review and meta-analysis.
Next Document:  Food choices made by low-income households when feeding their pre-school children: a qualitative stu...