Document Detail

Storage of human oocytes in the vapor phase of nitrogen.
MedLine Citation:
PMID:  20138272     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN). DESIGN: Prospective randomized study. SETTING: Private infertility center, IVI, Valencia. PATIENT(S): Oocyte donors (n = 44) and recipients (n = 46). INTERVENTION(S): Vitrification by the Cryotop method. Storage of vitrified oocytes in a vapor-phase nitrogen storage freezer and a traditional LN storage tank. Donation of the surviving oocytes and evaluation of fertilization, embryo development, and clinical results. MAIN OUTCOME MEASURE(S): Survival, fertilization, and cleavage rates. Embryo quality and clinical outcome. RESULT(S): Survival was 95.3% (vapor-phase nitrogen) and 94.5% (LN). Fertilization rates (73.1% and 71.7%) or cleavage on day 2 (95.6% and 94.7%), day 3 (84.5% and 79.9%), and blastocyst formation (54.7% and 53.9%) were similar between vapor-phase nitrogen and LN. Implantation, clinical, and ongoing pregnancy rates were similar for vapor-phase nitrogen (40.5%, 58.1%, and 48.8%, respectively) and LN groups (33.7%, 53.3%, and 46.6%, respectively). CONCLUSION(S): The vapor-phase nitrogen system permits the storage of oocytes vitrified, maintaining their potential to develop into competent embryos in a similar manner as those stored in a traditional LN freezer. This approach represents a practical alternative that prevents cross-contamination during the storage of vitrified samples.
Ana Cobo; Josep Ll Romero; Sonia Pérez; María J de los Santos; Marcos Meseguer; José Remohí
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Fertility and sterility     Volume:  94     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  2010-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1903-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
IVI-Universidad de Valencia, Valencia, Spain.
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MeSH Terms
Cell Division / physiology
Cell Survival / physiology
Cryopreservation / methods*
Embryo Transfer
Fertilization in Vitro / methods*
Infertility, Female / therapy
Middle Aged
Oocytes / physiology*
Pregnancy Rate
Prospective Studies
Treatment Outcome
Reg. No./Substance:

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