| Storage of human oocytes in the vapor phase of nitrogen. | |
| | |
MedLine Citation:
|
PMID: 20138272 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To evaluate the effectiveness of long-term vapor-phase nitrogen storage of vitrified human oocytes as a strategy for preventing the risk of cross-contamination due to direct contact with the liquid nitrogen (LN). DESIGN: Prospective randomized study. SETTING: Private infertility center, IVI, Valencia. PATIENT(S): Oocyte donors (n = 44) and recipients (n = 46). INTERVENTION(S): Vitrification by the Cryotop method. Storage of vitrified oocytes in a vapor-phase nitrogen storage freezer and a traditional LN storage tank. Donation of the surviving oocytes and evaluation of fertilization, embryo development, and clinical results. MAIN OUTCOME MEASURE(S): Survival, fertilization, and cleavage rates. Embryo quality and clinical outcome. RESULT(S): Survival was 95.3% (vapor-phase nitrogen) and 94.5% (LN). Fertilization rates (73.1% and 71.7%) or cleavage on day 2 (95.6% and 94.7%), day 3 (84.5% and 79.9%), and blastocyst formation (54.7% and 53.9%) were similar between vapor-phase nitrogen and LN. Implantation, clinical, and ongoing pregnancy rates were similar for vapor-phase nitrogen (40.5%, 58.1%, and 48.8%, respectively) and LN groups (33.7%, 53.3%, and 46.6%, respectively). CONCLUSION(S): The vapor-phase nitrogen system permits the storage of oocytes vitrified, maintaining their potential to develop into competent embryos in a similar manner as those stored in a traditional LN freezer. This approach represents a practical alternative that prevents cross-contamination during the storage of vitrified samples. |
| | |
Authors:
|
Ana Cobo; Josep Ll Romero; Sonia Pérez; María J de los Santos; Marcos Meseguer; José Remohí |
Publication Detail:
|
Type: Journal Article; Randomized Controlled Trial |
Journal Detail:
|
Title: Fertility and sterility Volume: 94 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-09-27 Completed Date: 2010-11-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
|
Languages: eng Pagination: 1903-7 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
IVI-Universidad de Valencia, Valencia, Spain. acobo@ivi.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cell Division / physiology Cell Survival / physiology Cryopreservation / methods* Embryo Transfer Female Fertilization in Vitro / methods* Humans Infertility, Female / therapy Middle Aged Nitrogen* Oocytes / physiology* Pregnancy Pregnancy Rate Prospective Studies Temperature* Treatment Outcome |
| Chemical | |
Reg. No./Substance:
|
7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genital schistosomiasis as a cause of female sterility and acute abdomen.
Next Document: The issue of reinfibulation.