Document Detail


Stomatobaculum longum gen. nov., sp. nov., an obligately anaerobic bacterium from the human oral cavity.
MedLine Citation:
PMID:  22843721     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A strictly anaerobic Gram-stain-variable but positive by structure, non-spore-forming bacterium designated Lachnospiraceae bacterium ACC2 strain DSM 24645(T) was isolated from human subgingival dental plaque. Bacterial cells were 4-40 µm long non-motile rods, often swollen and forming curved filaments up to 200 µm. Cells contained intracellular, poorly crystalline, nanometre-sized iron- and sulfur-rich particles. The micro-organism was able to grow on yeast extract, trypticase peptone, milk, some sugars and organic acids. The major metabolic end-products of glucose fermentation were butyrate, lactate, isovalerate and acetate. The growth temperature and pH ranges were 30-42 °C and 4.9-7.5, respectively. Major fatty acids were C14 : 0, C14 : 0 DMA (dimethyl aldehyde), C16 : 0, C16 : 1ω7c DMA. The whole-cell hydrolysate contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. The DNA G+C content was calculated to be 55.05 mol% from the whole-genome sequence and 55.3 mol% as determined by HPLC. There were no predicted genes responsible for biosynthesis of respiratory lipoquinones, mycolic acids and lipopolysaccharides. Genes associated with synthesis of teichoic and lipoteichoic acids, diaminopimelic acid, polar lipids and polyamines were present. According to the 16S rRNA gene sequence phylogeny, strain DSM 24645(T) formed, together with several uncultured oral clones, a separate branch within the family Lachnospiraceae, with the highest sequence similarity to the type strain of Moryella indoligenes at 94.2 %. Based on distinct phenotypic and genotypic characteristics, we suggest that strain DSM 24645(T) represents a novel species in a new genus, for which the name Stomatobaculum longum gen. nov., sp. nov. is proposed. The type strain of Stomatobaculum longum is DSM 24645(T) ( = HM-480(T); deposited in BEI Resources, an NIH collection managed by the ATCC).
Authors:
Maria V Sizova; Paul Muller; Nicolai Panikov; Manolis Mandalakis; Tine Hohmann; Amanda Hazen; William Fowle; Tanya Prozorov; Dennis A Bazylinski; Slava S Epstein
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-27
Journal Detail:
Title:  International journal of systematic and evolutionary microbiology     Volume:  63     ISSN:  1466-5034     ISO Abbreviation:  Int. J. Syst. Evol. Microbiol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-12     Completed Date:  2013-06-18     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  100899600     Medline TA:  Int J Syst Evol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1450-6     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AGEL01000000;  HM120209
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacteria, Anaerobic / classification,  genetics,  isolation & purification
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Dental Plaque / microbiology*
Fatty Acids / analysis
Female
Humans
Molecular Sequence Data
Mouth / microbiology*
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Grant Support
ID/Acronym/Agency:
1RC1DE020707-01/DE/NIDCR NIH HHS; 3 R21 DE018026-02S1/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Fatty Acids; 0/RNA, Ribosomal, 16S
Comments/Corrections

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