| Stoichiometry of 7-ethoxycoumarin metabolism by cytochrome P450 2B1 wild-type and five active-site mutants. | |
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MedLine Citation:
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PMID: 9369237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant P450 2B1 wild-type and the active-site mutants I114V, F206L, V363A, V363L, and G478S were purified and studied. The efficiency of coupling of reducing equivalents to 7-hydroxycoumarin formation was decreased for all the mutants except I114V. Uncoupling to H2O was increased for F206L, V363A, and G478S, decreased for V363L, and unchanged for I114V. Uncoupling to H2O2 was increased for V363L and decreased for I114V, F206L, and V363A. The findings from this study provide firm biochemical evidence that residues 206, 363, and 478 comprise part of the substrate binding site of P450 2B1. |
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Authors:
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X Fang; Y Kobayashi; J R Halpert |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 416 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-12-04 Completed Date: 1997-12-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 77-80 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, College of Pharmacy, The University of Arizona, Tucson 85721, USA. xfang@ccit.arizona.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Binding Sites Coumarins / metabolism* Cytochrome P-450 CYP2B1 / genetics, metabolism* Hydrogen Peroxide / metabolism Methylation Mutation NADP / metabolism Recombinant Proteins / genetics, metabolism Water / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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ES03619/ES/NIEHS NIH HHS; ES06694/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Coumarins; 0/Recombinant Proteins; 31005-02-4/7-ethoxycoumarin; 53-59-8/NADP; 7722-84-1/Hydrogen Peroxide; 7732-18-5/Water; EC 1.14.14.1/Cytochrome P-450 CYP2B1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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