| Stoichiometric conversion of biodiesel derived crude glycerol to hydrogen: Response surface methodology study of the effects of light intensity and crude glycerol and glutamate concentration. | |
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MedLine Citation:
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PMID: 22206915 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Photofermentation by the photosynthetic bacterium Rhodopseudomonas palustris has been used to convert the crude glycerol fraction from biodiesel production to hydrogen as a means of converting this large resource to useful energy. In the present study response surface methodology was applied to investigate the interactive effects among several important process parameters; light intensity, and the concentrations of crude glycerol and glutamate, on the stoichiometric conversion of crude glycerol to hydrogen. Under optimal conditions, a light intensity of 175W/m(2), 30mM glycerol, and 4.5mM glutamate, 6.69mol hydrogen/mole of crude glycerol were obtained, a yield 96% of theoretical. Determination of nitrogenase activity and expression levels showed that there was relatively little variation in levels of nitrogenase protein with changes in process variables whereas nitrogenase activity varied considerably, with maximal nitrogenase activity (228nmol of C(2)H(4)/ml/min) at the optimal central point. |
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Authors:
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Dipankar Ghosh; Irma Flore Sobro; Patrick C Hallenbeck |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-13 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Département de Microbiologie et Immunologie, Université de Montréal, CP 6128 Succursale Centre-ville, Montréal, Québec, Canada H3C 3J7. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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