Document Detail

Stochastic humoral expression of human growth hormone epitopes.
MedLine Citation:
PMID:  2452789     Owner:  NLM     Status:  MEDLINE    
Competition experiments between insolubilized monoclonal antibodies (mAb) and polyclonal antisera has led to the description of the humoral expression of human growth hormone (hGH) epitopes. This study was carried out with sera from mice and hamsters submitted to different immunization schedules: chronic administration of the antigen, secondary response and conventional hyperimmunization. The results indicated the absence of a unique immunodominant epitope in hGH; a significant individual variation of antibody (Ab) population titres with time; changes with time in the relative proportion of one Ab population with respect to the others; and the occurrence of Ab enhancing the 125I-hGH binding to five mAb depending upon the individuals and the time of immunization. Heterocliticity towards non-human GH was also detected. Although most of the animals showed cross-reacting Ab, two out of 12 mice, chronically injected, developed heteroclitic Ab. The data suggest that the humoral response to different epitopes of a protein antigen during the maturation of the immune response is a stochastic process leading to transient humoral immunodominance, enhancing Ab populations and heterocliticity, depending upon individual characteristics, either in outbred or inbred populations.
M Etcheverrigaray; A C Paladini; L A Retegui
Related Documents :
10628819 - Idiotype cascades associated with the cd4-hiv glycoprotein 120 interaction: immunizatio...
118999 - A solid-phase radioimmunoassay for the measurement of antibody to aspergillus in invasi...
25189679 - Therapeutic approaches in antibody-associated central nervous system pathologies.
1698629 - Alteration of the humoral immune response against muscle acetylcholine receptor by time...
1545119 - A pathogenic monoclonal antibody, g8, is characteristic of antierythrocyte autoantibodi...
3349649 - An idiotope-specific autoantibody in sle.
2211989 - On the mechanism of high-dose intravenous immunoglobulin treatment of patients with chr...
6777289 - Specificity of genes controlling immune responsiveness to (t,g)-a--l and (phe,g)-a--l.
1360879 - Cd4-independent binding of hiv-1 to the b lymphocyte receptor cr2 (cd21) in the presenc...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  63     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-06-23     Completed Date:  1988-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  595-601     Citation Subset:  IM    
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Junín, Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal / diagnostic use
Antibody Formation
Epitopes / immunology*
Growth Hormone / immunology*
Immune Sera / immunology
Immunization Schedule
Immunologic Memory
Mice, Inbred BALB C
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Immune Sera; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Human and murine antibodies to rye grass pollen allergen LolpIV share a common idiotope.
Next Document:  In vivo immunomodulation by the neuropeptide substance P.