Document Detail


Stochastic humoral expression of human growth hormone epitopes.
MedLine Citation:
PMID:  2452789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Competition experiments between insolubilized monoclonal antibodies (mAb) and polyclonal antisera has led to the description of the humoral expression of human growth hormone (hGH) epitopes. This study was carried out with sera from mice and hamsters submitted to different immunization schedules: chronic administration of the antigen, secondary response and conventional hyperimmunization. The results indicated the absence of a unique immunodominant epitope in hGH; a significant individual variation of antibody (Ab) population titres with time; changes with time in the relative proportion of one Ab population with respect to the others; and the occurrence of Ab enhancing the 125I-hGH binding to five mAb depending upon the individuals and the time of immunization. Heterocliticity towards non-human GH was also detected. Although most of the animals showed cross-reacting Ab, two out of 12 mice, chronically injected, developed heteroclitic Ab. The data suggest that the humoral response to different epitopes of a protein antigen during the maturation of the immune response is a stochastic process leading to transient humoral immunodominance, enhancing Ab populations and heterocliticity, depending upon individual characteristics, either in outbred or inbred populations.
Authors:
M Etcheverrigaray; A C Paladini; L A Retegui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  63     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-06-23     Completed Date:  1988-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  595-601     Citation Subset:  IM    
Affiliation:
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Junín, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / diagnostic use
Antibody Formation
Cricetinae
Epitopes / immunology*
Female
Growth Hormone / immunology*
Humans
Immune Sera / immunology
Immunization Schedule
Immunologic Memory
Mice
Mice, Inbred BALB C
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Immune Sera; 9002-72-6/Growth Hormone
Comments/Corrections

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