Document Detail


Stimulus-specific defect in the phagocytic pathways of annexin 1 null macrophages.
MedLine Citation:
PMID:  15197108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of the glucocorticoid-regulated protein annexin 1 during the process of phagocytosis has been studied using annexin 1 null peritoneal macrophages. Wild type and annexin 1 null macrophages were incubated with several distinct phagocytic targets. No differences were observed in rate or the maximal response with respect to IgG complexes or opsonised zymosan phagocytosis, as assessed by monitoring the production of reactive oxygen species. When annexin 1 null macrophages were incubated with non-opsonised zymosan particles, they exhibited impaired generation of reactive oxygen species, which was linked to a defect in binding of cells to the particles, as determined with fluorescent zymosan. This phenomenon was further confirmed by electron microscopy analysis, where annexin 1 null macrophages internalised fewer non-opsonised zymosan particles. Specific alterations in macrophage plasma membrane markers were observed in the annexin 1 null cells. Whereas no differences in dectin-1 and FcgammaR II/III expression were measured between the two genotypes, decreased membrane CD11b and F4/80 levels were measured selectively in macrophages lacking annexin 1. These cells also responded with an enhanced release of PGE(2) and COX-2 protein expression following addition of the soluble stimulants, LPS and heat-activated IgG. In conclusion, these results suggest that participation of endogenous annexin 1 during zymosan phagocytosis is critical and that this protein plays a tonic inhibitory role during macrophage activation.
Authors:
Simon Yona; Julia C Buckingham; Mauro Perretti; Roderick J Flower
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-06-14
Journal Detail:
Title:  British journal of pharmacology     Volume:  142     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-02     Completed Date:  2005-02-28     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  890-8     Citation Subset:  IM    
Affiliation:
Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary, University of London, Charterhouse Square, EC1 M 6BQ. simonyona@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A1 / genetics,  physiology*
Cell Membrane / metabolism,  ultrastructure
Dinoprostone / metabolism
Immunoglobulin G / pharmacology
Lipopolysaccharides / pharmacology
Macrophage Activation / drug effects,  genetics,  physiology
Macrophages / metabolism*,  ultrastructure
Mice
Mice, Knockout
Phagocytosis / genetics,  physiology*
Receptors, Cell Surface / metabolism
Signal Transduction / drug effects
Zymosan / metabolism
Chemical
Reg. No./Substance:
0/Annexin A1; 0/Immunoglobulin G; 0/Lipopolysaccharides; 0/Receptors, Cell Surface; 363-24-6/Dinoprostone; 9010-72-4/Zymosan
Comments/Corrections

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