Document Detail

Stimulatory effects of low-concentration reactive oxygen species on calcification ability of human dental pulp cells.
MedLine Citation:
PMID:  19084128     Owner:  NLM     Status:  MEDLINE    
The present study was conducted to investigate the effects of reactive oxygen species (ROS) on the calcification ability of human dental pulp (HDP) cells. HDP cells were treated with 100 mumol/L hydrogen peroxide (H(2)O(2)) for 5 or 10 minutes (5-min ROS group and 10-min ROS group) to investigate the mechanism of transmission to cells. Untreated cells were used as controls. Generation of free radicals was quantified by the electron spin resonance spin-trapping method and found to be increased by treatment with ROS. Formation of calcified nodules was also investigated by von Kossa staining and alizarin red S staining. Twenty-eight days after exposure, calcified nodules were present in cell cultures that had been treated with ROS for 5 or 10 minutes. Expression of mRNAs for osteopontin (OPN) and osteocalcin (OCN) was significantly greater in 10-min ROS group 6 and 9 days, respectively, after exposure than in controls. Production of OPN and OCN by 10-min ROS group was also greater 12 and 18 days, respectively, after exposure than in controls. These results suggested that calcification of HDP cells was stimulated by H(2)O(2) and by the ROS it generated.
Satoshi Matsui; Chitaka Takahashi; Yasuhisa Tsujimoto; Kiyoshi Matsushima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of endodontics     Volume:  35     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-03-09     Revised Date:  2009-05-21    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-72     Citation Subset:  D    
Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
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MeSH Terms
Calcification, Physiologic / drug effects*
Cell Differentiation / drug effects
Cells, Cultured
Dental Pulp / cytology,  drug effects*,  metabolism
Dentin, Secondary / secretion*
Hydrogen Peroxide / pharmacology
Osteoblasts / metabolism
Osteocalcin / biosynthesis
Osteopontin / biosynthesis
RNA, Messenger / biosynthesis
Reactive Oxygen Species / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Spin Trapping
Reg. No./Substance:
0/RNA, Messenger; 0/Reactive Oxygen Species; 104982-03-8/Osteocalcin; 106441-73-0/Osteopontin; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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