Document Detail

Stimulatory effect of zinc-chelating dipeptide on deoxyribonucleic acid synthesis in osteoblastic MC3T3-E1 cells.
MedLine Citation:
PMID:  8959758     Owner:  NLM     Status:  MEDLINE    
Whether deoxyribonucleic acid (DNA) synthesis in osteoblastic MC3T3-E1 cells is stimulated by zinc, an activator of bone formation, was investigated in vitro. After subculture for 3 days, the cells were cultured for up to 3 days (72 h) with zinc sulfate or zinc-chelated dipeptide (beta-alanyl-L-histidinato zinc; AHZ) in the range of 10(-7) to 10(-5) M. The culture with zinc compounds (10(-5) M) produced a significant increase of cell number, DNA content, and protein concentration in the cells, as reported previously. The culture with zinc compounds (10(-6) and 10(-5) M) clearly stimulated DNA synthesis in the homogenate, when it was estimated by the incorporation of [3H]deoxythymidine 5'-triphosphate into the DNA in the homogenate of cells. The AHZ effect was greater than that of zinc sulfate. The culture together with cycloheximide (19(-6) M) completely abolished the zinc compounds (10(-5) M)-induced increase of DNA synthesis in the cells, suggesting that the zinc compound effect is based on a newly synthesized protein component. Moreover, when zinc sulfate (10(-7) and 10(-6) M) or AHZ (10(-8) to 10(-5) M) was added into the reaction mixture with the homogenate of cells cultured without zinc compounds, the DNA synthesis was clearly increased. The effect of addition of zinc compounds (10(-6) M) on the DNA synthesis was completely inhibited by the presence of staurosporine (10(-8) M), an inhibitor of protein kinase C, or okadaic acid (10(-7) M), an inhibitor of protein phosphatase. The present study demonstrates that zinc compounds have a stimulatory effect on DNA synthesis in osteoblastic cells.
M Yamaguchi; T Matsui
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Peptides     Volume:  17     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  1996  
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1207-11     Citation Subset:  IM    
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
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MeSH Terms
Cell Division / drug effects
Cell Line
Chelating Agents / pharmacology*
DNA Replication / drug effects*
Dipeptides / pharmacology*
Organometallic Compounds / pharmacology*
Osteoblasts / cytology*
Reg. No./Substance:
0/Chelating Agents; 0/Dipeptides; 0/Organometallic Compounds; 0/di(N-acetylalanyl-histidinato)zinc

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