| Stimulatory effect of phospholipase A2 treatment on glucose utilization in human erythrocytes. | |
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MedLine Citation:
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PMID: 8418896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined whether modification of membrane phospholipids of human erythrocytes by hydrolysis with phospholipase A2 (PLA2 from bee venom) would affect glucose utilization, chosen as a typical model of intracellular metabolism, and, if so, intended to clarify the mechanism of the alteration of glycolysis. Treatment of erythrocytes with PLA2 induced a marked shape change (i.e., crenation) and significantly increased the rate of lactate production from glucose. Available evidence indicated that there is no relevance of this cell-shape change to the alteration of glycolysis. The lack of a detectable effect of papain treatment on glycolysis in PLA2-treated cells suggested that the increase in glycolysis by PLA2 treatment might not be caused by the conformational change of band-3 protein through modulation of membrane phospholipids. The result of the measurement of lactate production in the presence and absence of ouabain did not support the idea that hydrolysis of phospholipids by PLA2 treatment makes plasma membranes leaky to Na+ and consequently enhances glycolysis through activation of Na+/K(+)-ATPase. The action of PLA2 on glycolysis was abolished by extraction of free fatty acids in the cell membrane with bovine serum albumin. Loading erythrocytes with free fatty acid (oleic acid, linoleic acid, or arachidonic acid) caused a significant increase in glycolysis. Analysis of glycolytic intermediates suggested that the enhancement of glycolysis was induced by activation of 6-phosphofructokinase. The data, thus, indicate that treatment of human erythrocytes with PLA2 significantly accelerates glucose utilization and suggest that the stimulation of glycolysis is caused by activation of 6-phosphofructokinase through liberation of free fatty acids of membrane phospholipids by PLA2. |
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Authors:
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I Miwa; Y J Duan; S Murase; H Fukatsu; A Tamura; J Okuda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1175 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-02-09 Completed Date: 1993-02-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 174-80 Citation Subset: IM |
Affiliation:
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Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Erythrocyte Membrane / drug effects, metabolism Erythrocytes / drug effects*, metabolism Fatty Acids, Nonesterified / pharmacology Female Glucose / metabolism* Glycolysis Humans Lactates / analysis Lactic Acid Male Middle Aged Papain / pharmacology Phospholipases A / pharmacology* Phospholipases A2 Serum Albumin, Bovine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; 0/Lactates; 0/Serum Albumin, Bovine; 50-21-5/Lactic Acid; 50-99-7/Glucose; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2; EC 3.4.22.2/Papain |
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