Document Detail

Stimulatory effect of bleomycin on the synthesis of acidic glycosaminoglycans in cultured fibroblasts derived from rat carrageenin granuloma.
MedLine Citation:
PMID:  61044     Owner:  NLM     Status:  MEDLINE    
Cultured fibroblasts derived from rat carrageenin granuloma were treated with bleomycin and the synthesis of hexosamine-containing substances was compared with that in control cells. Four day treatment with o.1 mug bleomycin/ml resulted in a significant increase of the production of these macromolecules by the cells, though DNA synthesis was remarkably inhibited at this dose of bleomycin. The stimulatory effect could be seen as early as the second day of bleomycin treatment, and was enhanced with increasing treatment time. Further fractionation of the hexosamine-containing substances revealed that synthesis of acidic glycosaminoglycans was more sensitive to bleomycin than that of glycoproteins, i.e., acidic glycosaminoglycans increased by 80% and glycoproteins by 53% after four day treatment with 0.1 mug bleomycin/ml. The increased components of acidic glycosaminoglycans included not only hyaluronic acid but also sulphated glycosaminoglycans. Collagen synthesis was increased by 23% by the same dose of bleomycin. N-Acetyl-beta-glucosaminidase, one of the degradation enzymes for acidic glycosaminoglycans released into the cultured medium, was decreased significantly by bleomycin.
K Otsuka; S I Murota; Y Mori
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  444     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-12-03     Completed Date:  1976-12-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  359-68     Citation Subset:  IM    
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MeSH Terms
Acetylglucosaminidase / metabolism
Bleomycin / pharmacology*
Cells, Cultured
Collagen / biosynthesis
DNA / biosynthesis
Dose-Response Relationship, Drug
Fibroblasts / metabolism*
Glucosamine / metabolism
Glycoproteins / biosynthesis
Glycosaminoglycans / biosynthesis*
Granuloma / chemically induced,  metabolism*
Hydroxyurea / pharmacology
Proline / metabolism
Sulfates / metabolism
Thymidine / metabolism
Reg. No./Substance:
0/Glycoproteins; 0/Glycosaminoglycans; 0/Sulfates; 11056-06-7/Bleomycin; 127-07-1/Hydroxyurea; 147-85-3/Proline; 3416-24-8/Glucosamine; 50-89-5/Thymidine; 9000-07-1/Carrageenan; 9007-34-5/Collagen; 9007-49-2/DNA; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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