| Stimulatory effect of LPS and feedback effect of PGE2 on IL-27 production. | |
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MedLine Citation:
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PMID: 21044121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin (IL)-27 is a new member of the IL-6/IL-12 family, composed of two subunits, the Epstein-Barr virus-induced gene 3 (EBI3) and p28 chains (p28), and produced by activated monocytes and dendritic cells. IL-27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune cells. However, the pro-inflammatory mechanisms of IL-27 are still not well known. In this study, we investigated the effect of lipopolysaccharide (LPS) on the production of IL-27. We found that LPS-stimulated IL-27 production was in a dose-dependent and time-dependent manner in THP-1 cells. We have also shown that IL-27 induced PGE2 production and COX-2 gene expression at the level of mRNA as well as protein. Moreover, we found feed back effect of PGE2 on the production of IL-27 in THP-1 cells. The results suggest that PGE2 significantly inhibits LPS-induced IL-27 production, without affecting basal IL-27 expression. Further experiment suggests that PGE2 and LPS regulate IL-27 through NF-κB pathway. Our findings may have wide implication for IL-27 in inflammatory diseases. |
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Authors:
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C L Zhu; Y H Cao; R Zhang; Y Song; W Y Liu; F Pan; Y Li; Y Zhu; F Liu; J G Wu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 72 ISSN: 1365-3083 ISO Abbreviation: Scand. J. Immunol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: England |
Other Details:
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Languages: eng Pagination: 469-75 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Scandinavian Journal of Immunology © 2010 Blackwell Publishing Ltd. |
Affiliation:
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Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cyclooxygenase 2 / metabolism* Dinoprostone / metabolism* Dose-Response Relationship, Immunologic Feedback, Physiological Humans Interleukin-17 / immunology, metabolism* Lipopolysaccharides / immunology* Monocytes / immunology |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-17; 0/Lipopolysaccharides; 363-24-6/Dinoprostone; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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