| Stimulation of uterine cell cytokine production by ovarian hormones. | |
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MedLine Citation:
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PMID: 10976808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: Although leukocytes do not possess significant numbers of ovarian steroid hormone receptors, their numbers in the endometrium vary consistently, relative to the menstrual cycle. The possibility that cell types within the endometrium express leukocyte-attracting genes in response to ovarian hormones was investigated. METHOD OF STUDY: Endometrial biopsies were collected 10 days post-leutinizing hormone surge; the cell types were separated and cultured individually for 5 days in the presence of increasing amounts of estrogen or progesterone. Following culture, RNA was collected from cells and reverse-transcription-polymerase chain reaction was used to determine relative levels of gene expression of monocyte chemotactic proteins (MCP)-1, -2, and -3, and interleukin (IL)-12 p35 and p40. RESULTS: Although both endometrial stroma and glands were able to make MCP mRNA, steady-state levels of gene expression did not vary significantly relative to hormone treatment. The same was found for the p35 molecule of the IL-12 gene; however, differences were observed for the p40 subunit. CONCLUSIONS: Within the human endometrium, chemokines other than MCP and IL-12 are most likely responsible for cycle-related leukocyte recruitment. |
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Authors:
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J A DeLoia; A M Stewart-Akers; J Brekosky; C Kubik |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 44 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-12-08 Completed Date: 2001-02-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 16-21 Citation Subset: IM |
Affiliation:
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Magee-Women's Research Institute and the Department of Obstetrics, Gynecology, University of Pittsburgh, Pennsylvania, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Chemokine CCL2 / genetics, metabolism Chemokine CCL7 Chemokine CCL8 Cytokines* Endometrium / cytology, drug effects, metabolism* Estrogens / pharmacology* Female Gene Expression Regulation Humans Interleukin-12 / genetics, metabolism* Monocyte Chemoattractant Proteins / genetics, metabolism* Progesterone / pharmacology* RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
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HD35826/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCL7 protein, human; 0/CCL8 protein, human; 0/Chemokine CCL2; 0/Chemokine CCL7; 0/Chemokine CCL8; 0/Cytokines; 0/Estrogens; 0/Monocyte Chemoattractant Proteins; 0/RNA, Messenger; 187348-17-0/Interleukin-12; 57-83-0/Progesterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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