Document Detail


Stimulation of type IV collagenase expression by linoleic acid in a metastatic human breast cancer cell line.
MedLine Citation:
PMID:  8124668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Linoleic acid (LA), an omega-6 fatty acid, enhanced the appearance of type IV collagenase activity in culture medium conditioned by the metastatic MDA-MB-435 human breast cancer cell line; this effect was maximal with 0.75 microgram/ml LA. Zymography showed an increase in the gelatinolytic 92 kDa metalloproteinase, a form associated with the metastatic phenotype, during culture in the presence of 0.75 microgram/ml LA. Indomethacin, 20 micrograms/ml, completely suppressed the stimulation of collagenase by LA, suggesting a role for the eicosanoids. The tumor cells expressed mRNA for both the 72 and 92 kDa isoforms of type IV collagenase. Basal levels of the 92 kDa mRNA were much higher; both were up-regulated by LA despite the absence of detectable 72 kDa activity in conditioned medium.
Authors:
X H Liu; D P Rose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  76     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-04-12     Completed Date:  1994-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  71-7     Citation Subset:  IM    
Affiliation:
Division of Nutrition and Endocrinology, American Health Foundation, Valhalla, New York 10595.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / enzymology*,  metabolism
Collagenases / genetics*,  metabolism
Eicosanoids / biosynthesis
Extracellular Space / enzymology
Gene Expression / drug effects,  genetics
Humans
Isoenzymes / genetics*,  metabolism
Linoleic Acid
Linoleic Acids / pharmacology*
Matrix Metalloproteinase 9
Neoplasm Metastasis
RNA, Messenger / genetics
Stimulation, Chemical
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Eicosanoids; 0/Isoenzymes; 0/Linoleic Acids; 0/RNA, Messenger; 2197-37-7/Linoleic Acid; EC 3.4.24.-/Collagenases; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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