| Stimulation of somatostatin expression in developing ciliary ganglion neurons by cells of the choroid layer. | |
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MedLine Citation:
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PMID: 1671409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An important component of neuronal development is the matching of neurotransmitter expression with the appropriate target cell. We have examined how peptide transmitter expression is controlled in a simple model system, the avian ciliary ganglion (CG). This parasympathetic ganglion contains 2 distinct types of neurons: choroid neurons, which project to vasculature in the eye's choroid layer and use somatostatin as a co-transmitter with ACh, and ciliary neurons, which innervate the ciliary body and iris and use ACh but no known peptide co-transmitter. We have found that the earliest developmental stage in which neurons with somatostatinlike immunoreactivity (SOM-IR) are consistently found in vivo is stage 30 (embryonic day 6.5), a time shortly after the extension of neurites to targets in the eye's choroid layer. In cell culture, CG neurons expressed SOM-IR in co-culture with choroid cells, but not when cultured with striated muscle myotubes or with ganglion non-neuronal cells. No significant differences in neuronal survival or in ChAT activity were observed under these different co-culture conditions, which suggests that somatostatin expression is independently regulated. The stimulation of somatostatin expression was also specific in that other neuropeptides commonly found in autonomic neurons [neuropeptide Y (NPY), substance P (SP), vasoactive intestinal polypeptide (VIP)] were not induced in the presence of choroid cells. The ability to stimulate SOM-IR was not contact dependent because a macromolecule of greater than or equal to 10 kDa in choroid-conditioned medium (ChCM) was found to stimulate somatostatin expression in a dosage-dependent fashion. The somatostatin-stimulating activity induced SOM-IR in more than 90% of CG neurons, as well as in retrogradely labeled ciliary neurons, which would not normally express SOM-IR. Thus, the expression of somatostatin in cultured CG neurons is regulated by a macromolecule produced by cells in the choroid layer, a target normally innervated in vivo by CG neurons expressing somatostatin. |
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Authors:
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J N Coulombe; R Nishi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 11 ISSN: 0270-6474 ISO Abbreviation: J. Neurosci. Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-03-08 Completed Date: 1991-03-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 553-62 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Anatomy, Oregon Health Sciences University, Portland 97201. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cells, Cultured Chick Embryo Choroid / metabolism*, physiology Culture Media Ganglia, Parasympathetic / cytology, metabolism* Immunohistochemistry Muscle, Smooth / metabolism Neurons / metabolism* Neuropeptides / metabolism Somatostatin / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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EY06178/EY/NEI NIH HHS; NS25767/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Culture Media; 0/Neuropeptides; 51110-01-1/Somatostatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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