Document Detail


Stimulation of the side population fraction of ATDC5 chondroprogenitors by hypoxia.
MedLine Citation:
PMID:  19524690     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transporters, abcb1a/b (mdr1a/b) and abcg2 (bcrp1) was quantified by RT-PCR. SP cells were characterized by the higher expression of abcb1a. The expression levels of abcb1b and abcg2 were higher than abcb1a. However, there was no significant difference between SP and non-SP fractions in the expression of abcb1b and abcg2. The telomeric repeat amplification protocol assay showed that SP cells tended to show lower telomerase activity than non-SP cells. Chondrogenic properties of ATDC5 cells derived from SP or non-SP were assessed by micromass culture. There were not significant differences between SP and non-SP derived cells in Alcian blue staining and sox9, Aggrecan, Col2a1 and SZP mRNA expression. The results demonstrate that the SP fraction was stimulated by hypoxia and chondrogenic properties of SP and non-SP fraction of ATDC5 cells were similar.
Authors:
Koshi N Kishimoto; Carol L Oxford; A Hari Reddi
Publication Detail:
Type:  Journal Article     Date:  2009-06-12
Journal Detail:
Title:  Cell biology international     Volume:  33     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-20     Completed Date:  2010-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1222-9     Citation Subset:  IM    
Affiliation:
Center for Tissue Regeneration and Repair, Department of Orthopaedic Surgery, School of Medicine, University of California at Davis, Sacramento, CA 95817, USA. ahreddi@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzimidazoles
Cell Differentiation
Cell Fractionation
Cell Hypoxia / physiology*
Cell Line, Tumor
Cell Proliferation
Chondrocytes / metabolism,  pathology*
Extracellular Matrix / metabolism
Fluorescent Dyes
Gene Expression
Mice
Oxygen / physiology
Stem Cells / metabolism,  pathology*
Telomerase / metabolism*
Chemical
Reg. No./Substance:
0/Benzimidazoles; 0/Fluorescent Dyes; 23491-52-3/HOE 33342; 7782-44-7/Oxygen; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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