Document Detail

Stimulation of release of adrenal catecholamine by adenosine 3':5'-cyclic monophosphate and theophylline in the absence of extracellular Ca 2+ .
MedLine Citation:
PMID:  4337240     Owner:  NLM     Status:  MEDLINE    
Stimulation of catecholamine release was studied in the isolated adrenal of the cat during retrograde perfusion. Theophylline, adenosine 3':5'-cyclic monophosphate (cyclic AMP), and dibutyryl-cyclic AMP stimulated catecholamine release in adrenal chromaffin tissue; adenosine, 2'-AMP, 3'-AMP, and 5'-AMP were ineffective. Addition of theophylline for 5-15 min had no effect on catecholamine release induced by KCl or nicotine, but it significantly increased the responses to cyclic AMP and its dibutyryl derivative. Glands perfused with Ca(2+)-free Locke's solution for 30-180 min rapidly lost their responsiveness to KCl or nicotine. In contrast, exposure to Ca(2+)-free medium for 180 min had no effect on secretory responses to either the cyclic nucleotides, themselves, or to methylxanthine-induced potentiation of cyclic AMP responses. Thus, dibutyryl-cyclic AMP, cyclic AMP, and theophylline do not require extracellular Ca(2+) to release adrenal catecholamines. They may act by translocating intracellular bound Ca(2+) or by a mechanism independent of calcium.
M J Peach
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Apr 
Date Detail:
Created Date:  1972-07-20     Completed Date:  1972-07-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  834-6     Citation Subset:  IM    
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MeSH Terms
Adenosine / pharmacology
Adenosine Monophosphate / pharmacology
Adrenal Glands / drug effects,  secretion*
Butyrates / pharmacology
Calcium / pharmacology*
Catecholamines / secretion*
Cyclic AMP / pharmacology*
Drug Synergism
Nicotine / pharmacology
Potassium Chloride / pharmacology
Stimulation, Chemical
Theophylline / pharmacology*
Time Factors
Xanthines / pharmacology
Reg. No./Substance:
0/Butyrates; 0/Catecholamines; 0/Xanthines; 54-11-5/Nicotine; 58-55-9/Theophylline; 58-61-7/Adenosine; 60-92-4/Cyclic AMP; 61-19-8/Adenosine Monophosphate; 7440-70-2/Calcium; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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