| Stimulation of a rat uterine stromal cell line in culture reveals a molecular switch for endocrine-dependent differentiation. | |
| | |
MedLine Citation:
|
PMID: 15642789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Differentiation of uterine stromal cells is critical for the establishment of pregnancy. This study had two purposes: (i) to validate the use of the UIII rat uterine stromal cell model for investigating mechanisms underlying decidual cell differentiation, and (ii) to use this cell model to identify a molecular switch for cellular entry into the decidual cell differentiation pathway. Quiescent rat uterine stromal cells were transfected with a 500 bp segment of the decidual prolactin-related protein (dPRP) promoter ligated to a luciferase reporter gene. Cells were incubated in low-serum medium, or in low-serum medium containing progesterone (1 microM), estradiol 17-beta (10 nM), cholera toxin (10 ng/ml) and interleukin-11 (10 ng/ml). Protein extracts were collected 48 h later and luciferase was measured in the cellular lysates. Cholera toxin and interleukin-11 stimulated luciferase expression (P< 0.05) and addition of sex steroids further increased (P< 0.05) dPRP promoter activity. Stromal cells did not proliferate (P< 0.05) under differentiation conditions. Deletion analysis of the dPRP promoter revealed maximal luciferase expression between -250 and -500 bp relative to the transcription start site. Comparison of cyclin E/Cdk2 activity between proliferating and differentiating cells showed a 3-fold increase (P< 0.05) at 12 h in differentiating cells. The results suggest that cyclin E/Cdk2 serves as a molecular switch for uterine stromal cell entry into the decidual cell differentiation pathway. |
| | |
Authors:
|
V Rider; T Potapova; G Dai; M J Soares |
Related Documents
:
|
9532009 - Paget's disease versus toker cell hyperplasia in a supernumerary nipple. 8621269 - Estrogen activates invasiveness of endometrial cancel cells to the interstitium. 11675689 - Thymoma with pseudosarcomatous stroma. 10388519 - Autocrine stimulation of human mammary carcinoma cell proliferation by human growth hor... 7251819 - Aromatization of androstenedione by human adipose tissue stromal cells in monolayer cul... 10377039 - Transcription factors ets1, ets2, and elf1 exhibit differential localization in human e... 22760199 - Sensitization of glioma cells by x-linked inhibitor of apoptosis protein knockdown. 15528189 - Rab13 mediates the continuous endocytic recycling of occludin to the cell surface. 7138189 - Somatosensory rhythms in the awake cat: a single unit exploration of their thalamic con... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of endocrinology Volume: 184 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 2005 Jan |
Date Detail:
|
Created Date: 2005-01-11 Completed Date: 2005-02-25 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
|
Languages: eng Pagination: 119-27 Citation Subset: IM |
Affiliation:
|
Department of Biology, Pittsburg State University, Pittsburg, Kansas 66762, USA. VRider@pittstate.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Differentiation Cell Division Cell Line Cholera Toxin / pharmacology Cyclin E / genetics, metabolism Estradiol / pharmacology Female Genes, Switch Interleukin-11 / pharmacology Luciferases / analysis, genetics Progesterone / pharmacology Prolactin / analogs & derivatives*, genetics*, metabolism Promoter Regions, Genetic Rats Stimulation, Chemical Stromal Cells / cytology*, metabolism Transfection / methods Uterus / cytology*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
HD-39878/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cyclin E; 0/Dtprp protein, rat; 0/Interleukin-11; 50-28-2/Estradiol; 57-83-0/Progesterone; 9002-62-4/Prolactin; 9012-63-9/Cholera Toxin; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evidence that androgens and oestrogens, as well as follicle-stimulating hormone, can alter Sertoli c...
Next Document: The molecular basis of tamoxifen induction of mouse uterine epithelial cell proliferation.