Document Detail

Stimulation of a rat uterine stromal cell line in culture reveals a molecular switch for endocrine-dependent differentiation.
MedLine Citation:
PMID:  15642789     Owner:  NLM     Status:  MEDLINE    
Differentiation of uterine stromal cells is critical for the establishment of pregnancy. This study had two purposes: (i) to validate the use of the UIII rat uterine stromal cell model for investigating mechanisms underlying decidual cell differentiation, and (ii) to use this cell model to identify a molecular switch for cellular entry into the decidual cell differentiation pathway. Quiescent rat uterine stromal cells were transfected with a 500 bp segment of the decidual prolactin-related protein (dPRP) promoter ligated to a luciferase reporter gene. Cells were incubated in low-serum medium, or in low-serum medium containing progesterone (1 microM), estradiol 17-beta (10 nM), cholera toxin (10 ng/ml) and interleukin-11 (10 ng/ml). Protein extracts were collected 48 h later and luciferase was measured in the cellular lysates. Cholera toxin and interleukin-11 stimulated luciferase expression (P< 0.05) and addition of sex steroids further increased (P< 0.05) dPRP promoter activity. Stromal cells did not proliferate (P< 0.05) under differentiation conditions. Deletion analysis of the dPRP promoter revealed maximal luciferase expression between -250 and -500 bp relative to the transcription start site. Comparison of cyclin E/Cdk2 activity between proliferating and differentiating cells showed a 3-fold increase (P< 0.05) at 12 h in differentiating cells. The results suggest that cyclin E/Cdk2 serves as a molecular switch for uterine stromal cell entry into the decidual cell differentiation pathway.
V Rider; T Potapova; G Dai; M J Soares
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  184     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-11     Completed Date:  2005-02-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  119-27     Citation Subset:  IM    
Department of Biology, Pittsburg State University, Pittsburg, Kansas 66762, USA.
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MeSH Terms
Cell Differentiation
Cell Division
Cell Line
Cholera Toxin / pharmacology
Cyclin E / genetics,  metabolism
Estradiol / pharmacology
Genes, Switch
Interleukin-11 / pharmacology
Luciferases / analysis,  genetics
Progesterone / pharmacology
Prolactin / analogs & derivatives*,  genetics*,  metabolism
Promoter Regions, Genetic
Stimulation, Chemical
Stromal Cells / cytology*,  metabolism
Transfection / methods
Uterus / cytology*,  metabolism
Grant Support
Reg. No./Substance:
0/Cyclin E; 0/Dtprp protein, rat; 0/Interleukin-11; 50-28-2/Estradiol; 57-83-0/Progesterone; 9002-62-4/Prolactin; 9012-63-9/Cholera Toxin; EC 1.13.12.-/Luciferases

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