Document Detail

Stimulation of rat Schwann cell proliferation by estradiol: synergism between the estrogen and cAMP.
MedLine Citation:
PMID:  8387406     Owner:  NLM     Status:  MEDLINE    
Schwann cells dissociated from sciatic nerves of 4- to 5-day-old rats were established in long-term primary cultures. After treatment with anti-Thy-1.1 and complement, pure Schwann cell cultures were obtained as confirmed by indirect immunofluorescence staining of the S-100 antigen. The effect of estradiol on Schwann cell proliferation was tested. The estrogen alone had no effect on cell multiplication, but in the presence of forskolin (5 microM) or dibutyryl cyclic AMP (dbcAMP, 1 microM), estradiol (100 nM) became a potent mitogen. This effect was specific since when the anti-estrogen ICI-164,384 (200 nM) was added to the cultures, it completely inhibited the mitogenic effect of estradiol. In the absence of the estrogen, ICI-164,384 did not inhibit dbcAMP-induced cell growth. The effect of progesterone was also tested in the presence of forskolin, and no influence on cell multiplication was seen. Specific and saturable binding sites for estradiol were measured in Schwann cells by whole cell assay after labeling cells at 37 degrees C with [3H]estradiol and about 3000 specific binding sites per cell were found. Treatment of the cultures with forskolin (5 microM) for 48 or 72 h, but not for shorter times, resulted in a two-fold increase in estradiol binding. Estrogen receptors were visualized inside Schwann cells with a monoclonal anti-ER antibody by indirect immunofluorescence staining. Double-label immunofluorescence of ER and S-100 showed that ER immunostaining was restricted to spindle-shaped Schwann cells expressing the S-100 antigen.(ABSTRACT TRUNCATED AT 250 WORDS)
I Jung-Testas; M Schumacher; H Bugnard; E E Baulieu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  72     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-10     Completed Date:  1993-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  282-90     Citation Subset:  IM    
Lab. Hormones, INSERM U33, Le Kremlin-Bicêtre, France.
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MeSH Terms
Cell Division / drug effects
Cells, Cultured
Cyclic AMP / physiology*
Estradiol / analogs & derivatives,  pharmacology*
Estrogen Antagonists / pharmacology
Polyunsaturated Alkamides
Rats, Sprague-Dawley
Schwann Cells / cytology*
Reg. No./Substance:
0/Estrogen Antagonists; 0/Polyunsaturated Alkamides; 50-28-2/Estradiol; 60-92-4/Cyclic AMP; 98007-99-9/ICI 164384

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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