Document Detail

Stimulation of prostaglandin production in rat thymic epithelial cells by protein kinase C mediated activation of phospholipase A2.
MedLine Citation:
PMID:  1417924     Owner:  NLM     Status:  MEDLINE    
Tumor promoter (phorbol-12-myristate-13-acetate:PMA) stimulates the production of prostaglandin E2 (PGE2) in a dose- and time-dependent manner in cloned rat thymic epithelial cells, TEA3A1. This stimulation of PGE2 production by PMA was blocked by pretreatment of cells with protein kinase C (PKC) inhibitor staurosporin and was abolished in PKC down modulated cells. PMA treatment significantly stimulated the release of arachidonic acid from the cells, but had no effect on arachidonic acid incorporation and on cyclooxygenase enzymatic activity of the cells. These results indicate that PMA stimulates PGE2 production through PKC mediated activation of phospholipase A2 (PLA2) in thymic epithelial cells.
P S Liu; L Sun; M Wen; J Hayashi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry international     Volume:  27     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-11-23     Completed Date:  1992-11-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  931-8     Citation Subset:  IM    
W. Alton Jones Cell Science Center, Lake Placid, NY 12946.
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MeSH Terms
Arachidonic Acid / metabolism*
Cells, Cultured
Dinoprostone / biosynthesis*
Enzyme Activation / physiology
Epithelium / metabolism
Phospholipases A / drug effects,  metabolism*
Phospholipases A2
Protein Kinase C / physiology*
Tetradecanoylphorbol Acetate / pharmacology*
Thymus Gland / metabolism*
Grant Support
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; EC Kinase C; EC 3.1.1.-/Phospholipases A; EC A2

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