Document Detail

Stimulation of prostaglandin E2 synthesis in cloned osteoblastic cells of mouse (MC3T3-E1) by epidermal growth factor.
MedLine Citation:
PMID:  3096986     Owner:  NLM     Status:  MEDLINE    
Prostaglandin (PG) E2, known as a bone-resorption factor, was released as a predominant arachidonate metabolite in the culture medium of an osteoblastic cell line cloned from mouse calvaria (MC3T3-E1). Epidermal growth factor (EGF) (10 ng/ml) prominently enhanced endogenous PGE2 synthesis, requiring the simultaneous presence of unidentified factor(s) contained in bovine serum. PGE2 synthesis increased after a lag phase for 1-2 h and reached a maximum level at about 3 h after EGF addition. EGF-stimulated PGE2 synthesis was almost completely blocked by 10 microM cycloheximide or 1 microM actinomycin D. Furthermore, when the cells were pretreated with EGF, the microsomes exhibited an increased activity of fatty acid cyclooxygenase (arachidonic acid----PGH2), whereas the activity of PGE synthase (PGH2----PGE2) remained unchanged. These results suggested an EGF-mediated induction of cyclooxygenase. Following increased PGE2 synthesis, DNA synthesis increased and alkaline phosphatase activity decreased in a slower response to EGF. PGE2 (above 0.1 microM) added to the cells could replace EGF. However, such effects of EGF on the osteoblasts could not be attributed totally to an autocrine function of PGE2 produced by stimulation with EGF because these effects of EGF were not abolished by indomethacin, which blocked the PGE2 synthesis.
K Yokota; M Kusaka; T Ohshima; S Yamamoto; N Kurihara; T Yoshino; M Kumegawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  261     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1986-12-24     Completed Date:  1986-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15410-5     Citation Subset:  IM    
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MeSH Terms
Alkaline Phosphatase / metabolism
Cell Line
Cycloheximide / pharmacology
DNA / biosynthesis
Dactinomycin / pharmacology
Enzyme Induction / drug effects
Epidermal Growth Factor / pharmacology*
Osteoblasts / drug effects,  metabolism*
Prostaglandin-Endoperoxide Synthases / biosynthesis
Prostaglandins E / biosynthesis*
Reg. No./Substance:
0/Prostaglandins E; 363-24-6/Dinoprostone; 50-76-0/Dactinomycin; 62229-50-9/Epidermal Growth Factor; 66-81-9/Cycloheximide; 9007-49-2/DNA; EC Synthases; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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