Document Detail


Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta.
MedLine Citation:
PMID:  8460667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We sought to relate norepinephrine (NE) stimulation of phosphatidic acid (PA) production to functional responses of rat aorta and pathways for PA production. The time course for changes in PA was closely related to Ca-dependent tonic responses in 42K efflux and contraction. NE (30 microM for 1 min) increased PA and reduced phosphatidylcholine (PC) and phosphatidylinositol (PI) based on Pi analyses and 32P labeling of phospholipids. The 32P-to-Pi ratio in PA (0.8 +/- 0.2, n = 13) was similar to PC (0.8 +/- 0.1, n = 14) but was significantly lower (P < 0.001) than PI (4.6 +/- 0.5, n = 14). The 32P-to-Pi ratio in PA was also lower (P < 0.02) than phosphatidylinositol phosphate and phosphatidylinositol bisphosphate. NE also increased [3H]PA twofold (P < 0.05) when PC was selectively labeled with [3H]myristic acid. These observations are more consistent with PA being formed from the hydrolysis of PC by phospholipase D (PLD) than by the phosphorylation of diacylglycerol produced by the action of phospholipase C. PLD was assayed by the formation of phosphatidylethanol (PEt) via a transphosphatidylation reaction with ethanol (half-maximal stimulation at 0.4-0.5% vol/vol). The time course for PLD stimulation by NE was similar to PA, with significant increases (P < 0.002) during 10 s to 30 min exposure. Once formed, PEt was degraded slowly, with a half time > 3 h. It is concluded that NE stimulates PLD in rat aorta, which forms a significant amount of PA from the hydrolysis of PC.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
A W Jones; S D Shukla; B B Geisbuhler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  264     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-29     Completed Date:  1993-04-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C609-16     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Missouri School of Medicine, Columbia 65212.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / drug effects,  enzymology*,  metabolism*
Male
Norepinephrine / pharmacology*
Phosphatidic Acids / metabolism*
Phospholipase D / metabolism*
Phosphorus Radioisotopes
Rats
Rats, Sprague-Dawley
Time Factors
Tritium
Grant Support
ID/Acronym/Agency:
DK-01782/DK/NIDDK NIH HHS; HL-15852/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phosphorus Radioisotopes; 10028-17-8/Tritium; 51-41-2/Norepinephrine; EC 3.1.4.4/Phospholipase D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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