Document Detail

Stimulation of phospholipase A2 by xanthine oxidase in the rat corpus luteum.
MedLine Citation:
PMID:  1337274     Owner:  NLM     Status:  MEDLINE    
The ability of the superoxide radical (SOR) generated by xanthine oxidase to activate phospholipase A2 (PLA2) was examined in microsomes prepared from luteinized rat ovaries. Treatment of microsomes with xanthine oxidase resulted in a rapid burst in SOR formation followed by an increase in PLA2 activity. Stimulation of PLA2 activity was dose related and similar in microsomes prepared from control or prostaglandin F2 alpha (PGF2 alpha)-treated rats. Activation was inhibited by the antioxidants, vitamin E and nordihydroguaiaretic acid, and by superoxide dismutase and catalase, which metabolize SOR and H2O2 to remove reactive oxygen species from the cell. The stimulation of PLA2 activity by xanthine oxidase was dependent upon the addition of calcium ions, and it was highest in samples in which cytosol was added to membranes. These results indicate that the SOR and/or H2O2 may mediate PLA2 activation, which may be involved in the luteolytic process.
X M Wu; M Sawada; J C Carlson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  47     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-03-10     Completed Date:  1993-03-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1053-8     Citation Subset:  IM    
Biology Department, University of Waterloo, Ontario, Canada.
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MeSH Terms
Acetophenones / pharmacology
Analysis of Variance
Calcium / pharmacology
Cell Membrane / metabolism
Corpus Luteum / enzymology*
Cytosol / metabolism
Dose-Response Relationship, Drug
Electron Spin Resonance Spectroscopy
Enzyme Inhibitors / pharmacology
Imidazoles / pharmacology
Microsomes / drug effects,  enzymology*
Nordihydroguaiaretic Acid / pharmacology
Phospholipases A / antagonists & inhibitors,  biosynthesis*
Phospholipases A2
Rats, Wistar
Xanthine Oxidase / pharmacology*
Reg. No./Substance:
0/Acetophenones; 0/Enzyme Inhibitors; 0/Imidazoles; 11062-77-4/Superoxides; 500-38-9/Nordihydroguaiaretic Acid; 57265-65-3/calmidazolium; 7440-70-2/Calcium; 99-73-0/4-bromophenacyl bromide; EC Oxidase; EC 3.1.1.-/Phospholipases A; EC A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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