Document Detail


Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin.
MedLine Citation:
PMID:  375988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thrombin rapidly induces the formation of labeled phosphatidic acid from platelets prelabeled with [17C]arachidonate or 32PO34- and specifically decreases by 50--75% the content of phosphatidylinositol. Ionophore A23187 also stimulates phosphatidate labeling, but less effectively than thrombin. This effect on phosphatidic acid is blocked by increasing the levels of cyclic AMP by preincubation with dibutyryl cyclic AMP, cyclic AMP-phosphodiesterase inhibitors or prostacyclin. Indomethacin and eicosatetraynoic acid do not alter the production of phosphatidate, indicating independence from cyclooxygenase or lipoxygenase products. Increased turnover of [14C]- or [32P]phosphatidate occurs within 2--5 s after platelet activation by thrombin and is observed before endogenous, 14C-labeled arachidonate can be detected. The rate of phosphatidate formation parallels the induced rate of serotonin release. Release of [3H]serotonin is not affected by eicosatetraynoic acid. Phosphatidate production reflects the generation of diacylglycerol by C-type phospholipase degradation of phosphatidylinositol. Diacylglycerol and phosphatidic acid may participate in the membrane modification related to the early changes in platelet shape, release reactions or aggregation which occur on stimulation.
Authors:
E G Lapetina; P Cuatrecasas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  573     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 May 
Date Detail:
Created Date:  1979-08-16     Completed Date:  1979-08-16     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  394-402     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acids / blood*
Blood Platelets / drug effects,  metabolism*
Calcimycin / pharmacology
Epoprostenol / pharmacology
Horses
Phosphatidic Acids / blood*
Platelet Aggregation / drug effects
Serotonin / blood*
Thrombin / antagonists & inhibitors,  pharmacology
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Phosphatidic Acids; 35121-78-9/Epoprostenol; 50-67-9/Serotonin; 52665-69-7/Calcimycin; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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