Document Detail

Stimulation of phosphatidic acid of calcium influx and cyclic GMP synthesis in neuroblastoma cells.
MedLine Citation:
PMID:  6270138     Owner:  NLM     Status:  MEDLINE    
Phosphatidic acid added to the medium markedly elevated intracellular cyclic GMP content in cultured neuroblastoma N1E 115 cells. There was a significant elevation of cyclic GMP with 1 micrograms/ml and a maximum (70-fold) elevation with 100 micrograms/ml of phosphatidic acid. Other natural phospholipids did not increase, or increased only slightly, the cyclic GMP content in the cells. The elevation of cyclic GMP content by phosphatidic acid was absolutely dependent on extracellular calcium. Phosphatidic acid stimulated the influx of calcium into neuroblastoma cells 2- to 5-fold. The pattern of the calcium influx induced by phosphatidic acid was comparable to that of cyclic GMP elevation. The stimulation of calcium influx by phosphatidic acid was also observed in cultured heart cells, indicating that phosphatidic acid acts as a calcium ionophore or opens a specific calcium-gate in a variety of cell membranes. Treatment of neuroblastoma cells with phospholipase C increased 32Pi labeling of phosphatidic acid, stimulated the influx of calcium, and elevated the cyclic GMP content in the cells. Thus exogenous as well as endogenous phosphatidic acid stimulates the translocation of calcium across cell membranes and, as a consequence, induces the synthesis of cyclic GMP in the neuroblastoma cells.
S Ohsako; T Deguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10945-8     Citation Subset:  IM    
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MeSH Terms
Calcium / metabolism*
Cell Line
Cyclic GMP / biosynthesis*
Myocardium / metabolism
Neoplasms, Experimental / metabolism
Neuroblastoma / metabolism*
Phosphatidic Acids / pharmacology*
Phospholipids / pharmacology
Structure-Activity Relationship
Type C Phospholipases / pharmacology
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phospholipids; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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