Document Detail

Stimulation of neurotrophic factor secretion from 1321N1 human astrocytoma cells by novel diterpenoids, scabronines A and G.
MedLine Citation:
PMID:  10323283     Owner:  NLM     Status:  MEDLINE    
Glial cells release neurotrophic factors that maintain neurons functionally. When rat pheochromocytoma cells (PC-12) were cultivated with the conditioned medium of human astrocytoma cells (1321N1) incubated with the new diterpenoids, scabronines A and G, isolated from Sarcodon scabrosus, they changed their morphology and there was neurite outgrowth. The scabronines increased the expression of mRNA for nerve growth factor (NGF), and the secretion of NGF from 1321N1 cells in a concentration-dependent manner. However, the enhanced neurite outgrowth produced by the conditioned media was slightly inhibited by NGF neutralizing antibody, and the concentration of NGF released in response to the scabronines was insufficient to cause differentiation. These results suggest that scabronines cause the secretion of other factors together with NGF from 1321N1 cells. The diterpenoids are useful drugs to clarify the mechanism of synthesis and secretion of neurotrophic factors.
Y Obara; N Nakahata; T Kita; Y Takaya; H Kobayashi; S Hosoi; F Kiuchi; T Ohta; Y Oshima; Y Ohizumi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmacology     Volume:  370     ISSN:  0014-2999     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-07-06     Completed Date:  1999-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
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MeSH Terms
Astrocytoma / metabolism*,  pathology
Diterpenes / pharmacology*
Enzyme-Linked Immunosorbent Assay
Nerve Growth Factors / drug effects*,  secretion
Nerve Tissue Proteins / secretion*
Structure-Activity Relationship
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Diterpenes; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/scabronine A; 0/scabronine G

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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