| Stimulation of incretin secretion by dietary lipid: is it dose dependent? | |
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MedLine Citation:
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PMID: 19520739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After the ingestion of nutrients, secretion of the incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) by the enteroendocrine cells increases rapidly. Previous studies have shown that oral ingestion of fat stimulates secretion of both incretins; however, it is unclear whether there is a dose-dependent relationship between the amount of lipid ingested and the secretion of the hormones in vivo. Recently, we found a higher concentration of the incretin hormones in intestinal lymph than in peripheral or portal plasma. We therefore used the lymph fistula rat model to test for a dose-dependent relationship between the secretion of GIP and GLP-1 and dietary lipid. Under isoflurane anesthesia, the major mesenteric lymphatic duct of male Sprague-Dawley rats was cannulated. Each animal received a single, intraduodenal bolus of saline or varying amounts of the fat emulsion Liposyn II (0.275, 0.55, 1.1, 2.2, and 4.4 kcal). Lymph was continuously collected for 3 h and analyzed for triglyceride, GIP, and GLP-1 content. In response to increasing lipid calories, secretion of triglyceride, GIP, and GLP-1 into lymph increased dose dependently. Interestingly, the response to changes in intraluminal lipid content was greater in GLP-1- than in GIP-secreting cells. The different sensitivities of the two cell types to changes in intestinal lipid support the concept that separate mechanisms may underlie lipid-induced GIP and GLP-1 secretion. Furthermore, we speculate that the increased sensitivity of GLP-1 to intestinal lipid content reflects the hormone's role in the ileal brake reflex. As lipid reaches the distal portion of the gut, GLP-1 is secreted in a dose-dependent manner to reduce intestinal motility and enhance proximal fat absorption. |
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Authors:
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Stephanie M Yoder; Qing Yang; Tammy L Kindel; Patrick Tso |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-06-11 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 297 ISSN: 1522-1547 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-22 Completed Date: 2009-08-25 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G299-305 Citation Subset: IM |
Affiliation:
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University of Cincinnati, Department of Pathology and Laboratory Medicine, ML 0507, Cincinnati, OH 45237, USA. yodersy@email.uc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dose-Response Relationship, Drug Duodenum / drug effects*, metabolism, secretion Enteroendocrine Cells / drug effects*, secretion Fat Emulsions, Intravenous / administration & dosage*, metabolism Feedback, Physiological Gastric Inhibitory Polypeptide / secretion* Gastrointestinal Motility / drug effects Glucagon-Like Peptide 1 / secretion* Incretins / secretion* Intestinal Absorption / drug effects Intubation, Gastrointestinal Lymph / drug effects*, secretion Male Rats Rats, Sprague-Dawley Time Factors Triglycerides / secretion |
| Grant Support | |
ID/Acronym/Agency:
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DK-056863/DK/NIDDK NIH HHS; DK-059360/DK/NIDDK NIH HHS; DK-082205/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fat Emulsions, Intravenous; 0/Incretins; 0/Triglycerides; 112353-79-4/liposyn II; 59392-49-3/Gastric Inhibitory Polypeptide; 89750-14-1/Glucagon-Like Peptide 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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