| Stimulation of in vitro activation and the acrosome reaction of hamster spermatozoa by catecholamines. | |
| | |
MedLine Citation:
|
PMID: 283405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Capacitation and the acrosome reaction of mammalian spermatozoa are essential for fertilization. In vitro results are presented that demonstrate that catecholamines stimulate activation (a whiplash flagellar movement characteristic of capacitated hamster spermatozoa) and the acrosome reaction. Protein-free ultrafiltrates of bovine adrenal cortex and medulla preparations stimulated motility, activation, and acrosome reactions of hamster spermatozoa in the presence of bovine serum albumin. The medulla preparation was more effective than the cortex preparation in the stimulation of activation and acrosome reactions. Epinephrine (0.5-50 muM) and norepinephrine (50.0 muM) in the presence of bovine serum albumin and a partially purified protein-free cortex preparation also stimulated activation and the acrosome reactions. Both activation and acrosome reactions in the presence of epinephrine were inhibited by the adrenergic antagonists phentolamine and propranolol, suggesting the involvement of alpha- and beta-adrenergic receptors in the stimulation of capacitation and the acrosome reaction. In addition, phenylephrine, an alpha-adrenergic agonist, was as potent as epinephrine in the stimulation of acrosome reactions, but activation was reduced. Isoproterenol, a beta-adrenergic agonist, was as potent as epinephrine in the stimulation of activation, but acrosome reactions were reduced. High percentages of both activation and acrosome reactions were observed only in the presence of epinephrine, norepinephrine, or phenylephrine and isoproterenol together. |
| | |
Authors:
|
L E Cornett; S Meizel |
Related Documents
:
|
19958205 - Rapid diagnosis of the alpha-thalassemia-1 southeast asian type deletion using a single... 7693745 - Detection of hepatitis c virus rna in serum, by combining reverse transcription and pol... 22494355 - A copper-catalyzed petasis reaction for the synthesis of tertiary amines and amino esters. 21115095 - Induction of oxidative dna damage by mesalamine in the presence of copper: a potential ... 15260345 - Inhibition of pyrite oxidation by a phospholipid in the presence of silicate. 22925765 - Design and stereoselective synthesis of a c-aryl furanoside as a conformationally const... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 75 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1978 Oct |
Date Detail:
|
Created Date: 1979-04-25 Completed Date: 1979-04-25 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 4954-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrosome
/
drug effects Adrenal Cortex / physiology Animals Cricetinae Epinephrine / pharmacology* Isoproterenol / pharmacology Male Medulla Oblongata / physiology Norepinephrine / pharmacology* Phentolamine / pharmacology Phenylephrine / pharmacology Propranolol / pharmacology Sperm Capacitation / drug effects* Spermatozoa / drug effects* |
| Chemical | |
Reg. No./Substance:
|
50-60-2/Phentolamine; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 525-66-6/Propranolol; 59-42-7/Phenylephrine; 7683-59-2/Isoproterenol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced muta...
Next Document: Mid-G1 marker protein(s) in 3T3 mouse fibroblast cells.