Document Detail

Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene.
MedLine Citation:
PMID:  10620348     Owner:  NLM     Status:  MEDLINE    
Carotenoids and retinoids stimulate gap junctional communication (GJC), thought to be related to cancer-preventive properties. Lycopene, a nonprovitamin A carotenoid and its possible oxidation product, acyclo-retinoic acid, were tested for their effect on GJC, on stabilization of connexin43 mRNA, and on the transactivation of the RAR-beta2-promoter in vitro. In human fetal skin fibroblasts, GJC was stimulated by lycopene and acyclo-retinoic acid. Lycopene was effective at a concentration of 0.1 microM, whereas higher amounts of acyclo-retinoic acid (1 microM) were needed for comparable stimulation. Stabilizing effects of acyclo-retinoic acid on the mRNA of connexin43 via elements located in the 3'-UTR were weak. In comparison to retinoic acid (0.1 microM), considerably higher concentrations of the acyclo analog (50 microM) were required for similar effects; lycopene (0.1 microM) was not active in this system. Likewise, unphysiologically high levels of acyclo-retinoic acid (50 microM) were necessary to transactivate the RAR-beta2 promoter. The data demonstrate that acyclo-retinoic acid is much less active than retinoic acid with respect to GJC and retinoid-related signaling. Therefore, we conclude that lycopene affects GJC independent of the formation of acyclo-retinoic acid.
W Stahl; J von Laar; H D Martin; T Emmerich; H Sies
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  373     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-09     Completed Date:  2000-02-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  271-4     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, D-40001, Germany.
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MeSH Terms
Carotenoids / pharmacology*
Cell Communication / drug effects*
Cell Line
Connexin 43 / genetics
Gap Junctions / drug effects*,  physiology*
Promoter Regions, Genetic
RNA, Messenger / genetics,  metabolism
Receptors, Retinoic Acid / genetics
Transcriptional Activation / drug effects
Tretinoin / pharmacology*
Reg. No./Substance:
0/Connexin 43; 0/RNA, Messenger; 0/Receptors, Retinoic Acid; 0/retinoic acid receptor beta; 302-79-4/Tretinoin; 36-88-4/Carotenoids; 502-65-8/lycopene

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