Document Detail

Stimulation of fibroblast proliferation by the plant cysteine protease CMS2MS2 is independent of its proteolytic activity and requires ERK activation.
MedLine Citation:
PMID:  19747075     Owner:  NLM     Status:  MEDLINE    
The cysteine protease CMS2MS2 from Carica candamarcensis latex has been shown to enhance proliferation of L929 fibroblast and to activate the extracellular signal-regulated protein kinase (ERK). In experiments with CMS2MS2 irreversibly inhibited by E-64, the proliferative effect on fibroblasts remains unaffected. ERK phosphorylation mediated by CMS2MS2 was abolished in the presence of PD 98059 or U0126, both MAPK cascade inhibitors. In addition, these inhibitors suppress the mitogenic activity of intact CMS2MS2 or CMS2MS2-E-64. Furthermore, ERK phosphorylation and the mitogenic effect are partially suppressed by a phospholipase C (PLC) inhibitor. These data suggest that the mitogenic effect of CMS2MS2 on fibroblasts is independent of its proteolytic activity, requires ERK phosphorylation, and involves activation of PLC.
Marco Túlio R Gomes; Andréia P Turchetti; Miriam T P Lopes; Carlos E Salas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry     Volume:  390     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-01-28     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1285-91     Citation Subset:  IM    
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, CEP 31270-901, Brazil.
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MeSH Terms
Butadienes / pharmacology
Carica / enzymology*
Cell Line
Cell Proliferation* / drug effects
Cysteine Proteases / pharmacology*
Enzyme Activation
Fibroblasts / cytology*,  enzymology*
Flavonoids / pharmacology
Leucine / analogs & derivatives,  pharmacology
Mitogen-Activated Protein Kinase 1 / metabolism*
Mitogen-Activated Protein Kinase 3 / metabolism*
Mitogens / pharmacology*
Nitriles / pharmacology
Protease Inhibitors / pharmacology
Protein Kinase Inhibitors / pharmacology
Type C Phospholipases / metabolism
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Butadienes; 0/Flavonoids; 0/Mitogens; 0/Nitriles; 0/Protease Inhibitors; 0/Protein Kinase Inhibitors; 0/U 0126; 61-90-5/Leucine; 66701-25-5/E 64; EC Protein Kinase 1; EC Protein Kinase 3; EC 3.1.4.-/Type C Phospholipases; EC 3.4.-/Cysteine Proteases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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