Document Detail


Stimulation of fatty acid synthesis in vitro by gonadotrophin-induced testicular ribonucleic acid.
MedLine Citation:
PMID:  5657447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RNA from testes of hypophysectomized rats treated with follicle-stimulating hormone and luteinizing hormone markedly stimulates in vitro the incorporation of acetate and malonate (as CoA derivatives) into polyunsaturated fatty acids. The system in vitro contains the components necessary for both protein and fatty acid synthesis. That the RNA is a hormone-induced messenger type that causes enzyme synthesis that then causes fatty acid synthesis is supported by the following observations: (1) the stimulation of RNA synthesis by follicle-stimulating hormone and luteinizing hormone is decreased by injection of the animals with actinomycin D; (2) puromycin in the system in vitro decreases the synthesis of polyunsaturated fatty acids; (3) the activity of the RNA preparation is destroyed by digestion with ribonuclease; in fact, the digest is inhibitory, which is a characteristic of messenger-RNA-mediated protein synthesis; (4) protein that might be denatured enzyme is virtually absent from the effective RNA preparations.
Authors:
A Goswami; J K Skipper; W L Williams
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  108     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1968 Jun 
Date Detail:
Created Date:  1968-08-14     Completed Date:  1968-08-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  147-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Isotopes
Centrifugation, Density Gradient
Chromatography, Gas
Coenzyme A / metabolism
Dactinomycin / pharmacology
Depression, Chemical
Enzyme Induction
Fatty Acids / biosynthesis*
Follicle Stimulating Hormone / antagonists & inhibitors,  pharmacology*
Hypophysectomy
Luteinizing Hormone / antagonists & inhibitors,  pharmacology*
Male
Microsomes / metabolism*
Orotic Acid / metabolism
Pituitary Gland / physiology
Protein Biosynthesis
Protein Denaturation
Puromycin / pharmacology
RNA / pharmacology*
RNA, Messenger
Rats
Testis / metabolism*
Tritium
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids; 0/RNA, Messenger; 10028-17-8/Tritium; 50-76-0/Dactinomycin; 53-79-2/Puromycin; 63231-63-0/RNA; 65-86-1/Orotic Acid; 85-61-0/Coenzyme A; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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